一种用于实验动物代谢研究中定量测定呼出14CO2的简单、快速且半自动的方法。
A simple, rapid and semi-automated method for the quantitative determination of expired 14CO2 in metabolism studies with laboratory animals.
作者信息
Goon D J, Nagasawa H T
出版信息
Res Commun Chem Pathol Pharmacol. 1977 Apr;16(4):745-8.
The automatic features of a commercial instrument which prepares 14CO2 for liquid scintillation counting have been adapted to assay the 14CO2 collected in aqueous NaOH from the expired air of experimental laboratory animals in metabolism experiments. The method involves the liberation of 14CO2 trapped as Na214CO3 with dilute H2SO4 and the entrainment of the 14CO2 with nitrogen into the sample handling system. Recoveries were 87.3+/-1.2% and precision+/-2.0%. The method was simple and rapid; 6-8 samples were processed in one hour.
一种用于制备用于液体闪烁计数的14CO2的商用仪器的自动功能，已被用于测定代谢实验中从实验动物呼出气体中收集在氢氧化钠水溶液中的14CO2。该方法包括用稀硫酸释放被困为Na214CO3的14CO2，并用氮气将14CO2带入样品处理系统。回收率为87.3±1.2%，精密度为±2.0%。该方法简单快速；一小时内可处理6-8个样品。
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