Nishikawa T, Suzuki S, Kubo H, Ohtani H
Department of Clinical Pathology, School of Medicine, Kitasato University, Kanagawa, Japan.
J Chromatogr A. 1996 Jan 12;720(1-2):167-72. doi: 10.1016/0021-9673(95)00280-4.
Four monosaccharides (glucose, galactose, mannose and fructose) and one disaccharide (maltose) were subjected to high-performance liquid chromatography with UV or refractive index detection. Various profiles such as broad, tailed and splitted peaks were produced, depending on column temperature and eluent flow-rate because these saccharides underwent isomerization. In contrast, alpha-methylglucoside, a non-converting derivative, always produced a sharp peak. By analyzing these profiles kinetic constants of the isomerization were obtained and compared with the literature data.
四种单糖（葡萄糖、半乳糖、甘露糖和果糖）和一种二糖（麦芽糖）通过配备紫外或示差折光检测的高效液相色谱进行分析。由于这些糖类会发生异构化，根据柱温和洗脱液流速的不同，会产生各种峰形，如宽峰、拖尾峰和分裂峰。相比之下，α-甲基葡萄糖苷这种不会发生转化的衍生物总是产生尖锐的峰。通过分析这些峰形，获得了异构化的动力学常数，并与文献数据进行了比较。
