Müthing J
Institute of Cell Culture Technology, Faculty of Technical Sciences, University of Bielefeld, Germany.
J Chromatogr A. 1996 Jan 12;720(1-2):3-25. doi: 10.1016/0021-9673(95)00499-8.
In this review an updated overview of current improvements on thin-layer chromatography (TLC) of gangliosides over the past decade is provided. Basic general techniques and special advice is given for successful separation of glycosphingolipids. New approaches concerning continuous and multiple development, and several preparative TLC methods are also included. Emphasis is placed on TLC immunostaining and related techniques, i.e. practical applications of carbohydrate-specific antibodies, toxins and bacteria, viruses, lectins and eukaryotic cells. Thus, this review on ganglioside TLC summarizes its power as an analytical tool for a wide range of purposes.
在本综述中，提供了过去十年间神经节苷脂薄层色谱（TLC）当前改进的最新概述。给出了成功分离糖鞘脂的基本通用技术和特别建议。还包括了关于连续和多次展开的新方法以及几种制备型TLC方法。重点在于TLC免疫染色及相关技术，即碳水化合物特异性抗体、毒素、细菌、病毒、凝集素和真核细胞的实际应用。因此，这篇关于神经节苷脂TLC的综述总结了其作为用于多种目的的分析工具的作用。
