Landgren E, Eriksson A, Wennström S, Kanda S, Claesson-Welsh L
Ludwig Institute for Cancer Research, Uppsala, Sweden.
Exp Cell Res. 1996 Mar 15;223(2):405-11. doi: 10.1006/excr.1996.0095.
Expression levels of growth factor receptors are subject to complex regulation, which is of consequence for their signaling capacity in physiological and pathological processes. We examined the regulation of expression levels of fibroblast growth factor receptor 1 (FGFR-1) in human fibroblasts treated with a panel of growth regulatory factors. Only platelet-derived growth factor BB (PDGF-BB) treatment had a significant effect and induced FGFR-1 mRNA levels fourfold, with a peak around 8 h of stimulation. The increase in mRNA levels was followed by an increased synthesis of FGFR-1 protein, which responded to basic FGF (bFGF) stimulation with induction of kinase activity and biological signaling. Thus, murine brain endothelial cells displayed an augmented induction of plasminogen activator activity in response to bFGF, following treatment with PDGF-BB. These data suggest that PDGF-BB could support FGFR-1-mediated biological responses in processes such as angiogenesis.
生长因子受体的表达水平受到复杂的调控，这对其在生理和病理过程中的信号传导能力具有重要意义。我们检测了一组生长调节因子处理的人成纤维细胞中，成纤维细胞生长因子受体1（FGFR-1）表达水平的调控情况。只有血小板衍生生长因子BB（PDGF-BB）处理有显著效果，可使FGFR-1 mRNA水平增加四倍，在刺激约8小时时达到峰值。mRNA水平增加后，FGFR-1蛋白的合成也增加，该蛋白对碱性成纤维细胞生长因子（bFGF）刺激有反应，可诱导激酶活性和生物信号传导。因此，在用PDGF-BB处理后，鼠脑内皮细胞对bFGF的反应显示出纤溶酶原激活物活性的增强诱导。这些数据表明，PDGF-BB在血管生成等过程中可能支持FGFR-1介导的生物学反应。
