Bobrysheva I V, Varshaver N B
Genetika. 1995 Dec;31(12):1598-604.
Chinese hamster cell clones of independent origin, which were resistant to purine base analogs and induced by the activated c-Ha-ras1 oncogene, were isolated. It was shown that the isolated clones stably retained resistance after cultivation on a medium without an analog, confirming mutational nature of the resistance. Most of the clones are able to grow on the HAT medium, retaining partial activity of the hypoxanthine phosphoribosyltransferase enzyme (HPRT); i.e., they are leaky mutants. Analysis by blot-hybridization did not reveal the presence of human ras-sequences in any of the mutants studied. Evidently, the mutagenic action of the oncogene is not insertional, and resistance is not linked to the stably integrated oncogene. The mutagenic effect of c-Ha-ras1 is likely to be of the "hit-and-run" type.
分离出了独立起源的中国仓鼠细胞克隆，这些克隆对嘌呤碱基类似物具有抗性，并且是由活化的c-Ha-ras1癌基因诱导产生的。结果表明，分离出的克隆在不含类似物的培养基上培养后仍稳定保留抗性，证实了抗性的突变性质。大多数克隆能够在HAT培养基上生长，保留次黄嘌呤磷酸核糖基转移酶（HPRT）的部分活性；也就是说，它们是渗漏突变体。通过印迹杂交分析，在所研究的任何突变体中均未发现人ras序列的存在。显然，癌基因的诱变作用不是插入性的，并且抗性与稳定整合的癌基因无关。c-Ha-ras1的诱变作用可能是“打了就跑”类型的。
