多药耐药相关蛋白基因在人软组织肉瘤中的表达
Expression of multidrug-resistance-associated protein gene in human soft-tissue sarcomas.
作者信息
Oda Y, Schneider-Stock R, Ryś J, Gruchala A, Niezabitowski A, Roessner A
机构信息
Institute of Pathology, Faculty of Medicine, Otto-von-Guericke University, Magdeburg, Germany.
出版信息
J Cancer Res Clin Oncol. 1996;122(3):161-65. doi: 10.1007/BF01366956.
We examined the mRNA expression of the multidrug- resistance-associated protein gene (MRP) in soft-tissue sarcomas and compared it with the expression of the multidrug resistance gene (MDR1), using the reverse transcriptase/polymerase chain reaction. We investigate 39 samples from 33 cases of soft-tissue sarcomas (11 liposarcomas, 9 malignant fibrous histiocytomas, 6 leiomyosarcomas, 4 malignant schwannomas, 3 fibrosarcomas, 3 synovial sarcomas, and 3 epithelioid sarcomas) and 7 benign soft-tissue tumors. All samples were obtained prior to chemotherapy. An expression of MRP mRNA was noted in 56% of soft-tissue sarcoma specimens. The co-expression of MRP and MDR1 was recognized in 15 samples (38%) (5/11 liposarcomas, 5/9 malignant fibrous histiocytomas, 3/6 leiomyosarcomas, 2/3 fibrosarcomas) and significantly correlated with histological grade (P=0.0165). A positive and significant correlation was found between MRP and MDR1 expression in soft-tissue sarcomas(P=0.0013). In benign soft-tissue tumors, 1 chemodectoma and 1 neurothekeoma showed low MRP expression; however, no case showed co-expression of MRP and MDR1.
我们使用逆转录酶/聚合酶链反应检测了软组织肉瘤中多药耐药相关蛋白基因（MRP）的mRNA表达，并将其与多药耐药基因（MDR1）的表达进行比较。我们研究了33例软组织肉瘤（11例脂肪肉瘤、9例恶性纤维组织细胞瘤、6例平滑肌肉瘤、4例恶性神经鞘瘤、3例纤维肉瘤、3例滑膜肉瘤和3例上皮样肉瘤）和7例良性软组织肿瘤的39个样本。所有样本均在化疗前获取。在56%的软组织肉瘤标本中检测到MRP mRNA表达。MRP和MDR1的共表达在15个样本（38%）中被识别（5/11脂肪肉瘤、5/9恶性纤维组织细胞瘤、3/6平滑肌肉瘤、2/3纤维肉瘤），且与组织学分级显著相关（P = 0.0165）。在软组织肉瘤中，MRP和MDR1表达之间存在正相关且具有显著性（P = 0.0013）。在良性软组织肿瘤中，1例化学感受器瘤和1例神经鞘黏液瘤显示出低水平的MRP表达；然而，没有病例显示MRP和MDR1的共表达。
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