单纯疱疹病毒2型HG52毒株的RL1基因含有一个154碱基对的内含子，这是根据序列分析预测得出的。

The herpes simplex virus type 2 strain HG52 RL1 gene contains a 154 bp intron as predicted from sequence analysis.

作者信息

Harland J E, Bdour S, Brown S M, MacLean A R

机构信息

MRC Virology Unit, Institute of Virology, Glasgow, UK.

出版信息

J Gen Virol. 1996 Mar;77 ( Pt 3):481-4. doi: 10.1099/0022-1317-77-3-481.

DOI:10.1099/0022-1317-77-3-481

PMID:8601785

Abstract

The published sequence of the herpes simplex virus (HSV) type 2 strain HG52 neurovirulence gene RL1 indicated that, unlike the HSV-1 homologue, the gene contains a 154 bp intron. This intron contains six complete and one partial copy of a 19 bp repeat which encodes a stop codon; thus all three forward frames are blocked. By RT-PCR of poly(A)+ RNA, using primers on either side of the proposed intron, we have isolated a partial cDNA clone corresponding to the predicted spliced mRNA.

摘要

已发表的单纯疱疹病毒2型（HSV-2）HG52株神经毒力基因RL1的序列表明，与HSV-1同源物不同，该基因含有一个154 bp的内含子。这个内含子包含一个19 bp重复序列的六个完整拷贝和一个部分拷贝，该重复序列编码一个终止密码子；因此所有三个正向读码框都被阻断。通过使用位于推测内含子两侧的引物对poly(A)+ RNA进行RT-PCR，我们分离出了一个与预测的剪接mRNA相对应的部分cDNA克隆。