Pigny P, Van Seuningen I, Desseyn J L, Nollet S, Porchet N, Laine A, Aubert J P
INSERM U 377, Lille, France.
Biochem Biophys Res Commun. 1996 Mar 7;220(1):186-91. doi: 10.1006/bbrc.1996.0378.
MUC5B gene is one of the four human mucin genes mapped to chromosome 11p15. The identification of three potential Sp1 binding sites located between the tandem repeat and the 3' end of MUC5B suggests a possible regulatory role for this region. In this report we show by electrophoretic mobility shift assay that only one potential Sp1 binding site (NAU62) leads to a specific interaction with a nuclear factor from HT-29 MTX cells which does not exist in parental HT-29 cells. By using mutated versions of NAU62, an 18 mer sequence within this later was shown to be directly involved in the interaction. The nuclear factor called NF1-MUC5B which binds to this element has a Mr of 42000 and is not Sp1. These results suggest that MUC5B contains a sequence in its 3' region that might act as a cis-element. This report opens the field of transcriptional regulation of human mucin genes encoding secreted mucins.
MUC5B基因是定位于11号染色体p15区域的四个人类黏蛋白基因之一。在MUC5B的串联重复序列与3'端之间鉴定出三个潜在的Sp1结合位点，提示该区域可能具有调控作用。在本报告中，我们通过电泳迁移率变动分析表明，只有一个潜在的Sp1结合位点（NAU62）可导致与HT - 29 MTX细胞的核因子发生特异性相互作用，而亲本HT - 29细胞中不存在这种相互作用。通过使用NAU62的突变体，结果显示该序列内的一个18聚体序列直接参与了这种相互作用。与该元件结合的核因子称为NF1 - MUC5B，其分子量为42000，并非Sp1。这些结果表明，MUC5B在其3'区域含有一个可能作为顺式元件的序列。本报告开启了编码分泌性黏蛋白的人类黏蛋白基因转录调控领域的研究。
