Rajagopalan-Levasseur P, Lecointe D, Bertrand G, Fay M, Gougerot-Pocidalo M A
Institut National de la Santé et de la Recherche Médicale, Hôpital Bichat-Claude Bernard, Paris, France.
Clin Exp Immunol. 1996 Apr;104(1):48-53. doi: 10.1046/j.1365-2249.1996.d01-644.x.
L-arginine-dependent reactive nitrogen intermediates have been identified as macrophage cytotoxic effector molecules against intracellular pathogens. To determine its role, ex vivo production of NO by peritoneal macrophages of C3H/HeN mice and Dunkin-Hartley guinea pigs infected intraperitoneally with a virulent and isogenic avirulent Legionella pneumophila serogroup 1 strain was compared with bacterial clearance from the lungs. While the virulent strain was cleared from mice lungs, the guinea pigs died within 96 h. In vivo infection with both strains resulted in the production of NO by mouse peritoneal macrophages ex vivo. In contrast, guinea pig macrophages did not produce detectable NO. In addition, infection by the avirulent strain led to the production of significantly more NO by mouse macrophages than the virulent parent strain, irrespective of stimulation with lipopolysaccharide (LP) and/or interferon-gamma (ifn-gamma). These results suggest that resistance to Leg. pneumophila infection may depend on the production of NO by host macrophages.
L-精氨酸依赖性反应性氮中间产物已被确定为巨噬细胞针对细胞内病原体的细胞毒性效应分子。为了确定其作用，将腹腔注射毒力和同基因无毒嗜肺军团菌血清1型菌株的C3H/HeN小鼠和Dunkin-Hartley豚鼠的腹腔巨噬细胞体外产生一氧化氮（NO）的情况与肺部细菌清除情况进行了比较。虽然毒力菌株从小鼠肺部清除，但豚鼠在96小时内死亡。用这两种菌株进行体内感染均导致小鼠腹腔巨噬细胞在体外产生NO。相比之下，豚鼠巨噬细胞未产生可检测到的NO。此外，无毒菌株感染导致小鼠巨噬细胞产生的NO比有毒亲本菌株显著更多，无论是否用脂多糖（LPS）和/或干扰素-γ（IFN-γ）刺激。这些结果表明，对嗜肺军团菌感染的抵抗力可能取决于宿主巨噬细胞产生NO的情况。
