Jepras R I, Fitzgeorge R B, Baskerville A
J Hyg (Lond). 1985 Aug;95(1):29-38. doi: 10.1017/s0022172400062252.
Two strains of Legionella pneumophila (LP) serogroup I, of differing virulence, were examined in terms of numbers of viable organisms in tissues, pyrexia and mortality following aerosol infection. The Corby strain was the more virulent, with pyrexia and deaths of guinea-pigs 3 to 6 days after infection. This strain multiplied very rapidly in the lungs to reach a peak of 5 X 10(11) viable organisms/lung. Organisms were present in the blood, liver, spleen and kidney. The Philadelphia-1 strain (NCTC 11192) was unable to replicate in the lung and was cleared between 14 and 21 days after infection. Pyrexia was not observed. No guinea-pigs died and viable LP was not found in any organ other than the lung. Lung lavages on aerosol infected animals were performed and the virulent Corby strain was found to be mainly intracellular. The avirulent Philadelphia-1 strain was found predominantly in the extracellular location. There were approximately 10 times the number of viable virulent LP in the lung macrophage fraction than in the lung PMNL fraction. In comparison, there were approximately equal numbers of the viable avirulent strain in the macrophages and the PMNL. Experimental evidence suggests that the macrophage preferentially supports the growth of the virulent Corby strain compared with the PMNL. The avirulent strain on the other hand appears to be destroyed by both the macrophages and the PMNL.
对两株毒力不同的嗜肺军团菌（LP）血清群I菌株进行了研究，观察了气溶胶感染后组织中活菌数量、发热情况及死亡率。科比菌株毒力更强，豚鼠在感染后3至6天出现发热和死亡。该菌株在肺中迅速繁殖，达到每肺5×10¹¹个活菌的峰值。血液、肝脏、脾脏和肾脏中均有细菌存在。费城-1菌株（NCTC 11192）在肺中无法复制，在感染后14至21天被清除。未观察到发热现象。没有豚鼠死亡，除肺部外，在其他任何器官中均未发现活菌LP。对气溶胶感染动物进行了肺灌洗，发现有毒力的科比菌株主要存在于细胞内。无毒力的费城-1菌株主要存在于细胞外。肺巨噬细胞部分中的活菌毒力LP数量约为肺中性粒细胞部分的10倍。相比之下，巨噬细胞和中性粒细胞中的无毒力菌株活菌数量大致相等。实验证据表明，与中性粒细胞相比，巨噬细胞更有利于有毒力的科比菌株生长。另一方面，无毒力菌株似乎会被巨噬细胞和中性粒细胞都破坏。
