Increased cloning efficiency by temperature-cycle ligation.
作者信息
Lund A H, Duch M, Pedersen F S
机构信息
Department of Molecular Biology, University of Aathus, Denmark.
出版信息
Nucleic Acids Res. 1996 Feb 15;24(4):800-1. doi: 10.1093/nar/24.4.800.
Abstract
摘要
相似文献
1
Increased cloning efficiency by temperature-cycle ligation.
Nucleic Acids Res. 1996 Feb 15;24(4):800-1. doi: 10.1093/nar/24.4.800.
2
Cloning of DNA fragments: ligation reactions in agarose gel.
Methods Mol Biol. 2014;1099:117-21. doi: 10.1007/978-1-62703-715-0_10.
3
A modified version of the digestion-ligation cloning method for more efficient molecular cloning.
Anal Biochem. 2014 May 15;453:55-7. doi: 10.1016/j.ab.2014.02.031. Epub 2014 Mar 5.
4
Optimizing T4 DNA polymerase conditions enhances the efficiency of one-step sequence- and ligation-independent cloning.
Biotechniques. 2017 Sep 1;63(3):125-130. doi: 10.2144/000114588.
5
Effect of growth temperature and vector ends phosphorylation in the cloning efficiency of specific DNA fragments.
Biotechniques. 1999 Jun;26(6):1022-4. doi: 10.2144/99266bm02.
6
A universal mini-vector and an annealing of PCR products (APP)-based cloning strategy for convenient molecular biological manipulations.
Biochem Biophys Res Commun. 2018 Mar 18;497(4):978-982. doi: 10.1016/j.bbrc.2018.02.099. Epub 2018 Feb 12.
7
Universal CG cloning of polymerase chain reaction products.
Anal Biochem. 2015 Feb 15;471:80-2. doi: 10.1016/j.ab.2014.10.018. Epub 2014 Nov 4.
8
Efficient cloning method that selects the recombinant clones.
Biotechniques. 1997 Aug;23(2):292-5. doi: 10.2144/97232st05.
9
SLIC: a method for sequence- and ligation-independent cloning.
Methods Mol Biol. 2012;852:51-9. doi: 10.1007/978-1-61779-564-0_5.
10
Efficient ligation and cloning of DNA fragments with 2-bp overhangs.
Anal Biochem. 2010 Jul 1;402(1):91-2. doi: 10.1016/j.ab.2010.03.020. Epub 2010 Mar 15.
引用本文的文献
1
Engineering a New IFN-ApoA-I Fusion Protein with Low Toxicity and Prolonged Action.
Molecules. 2023 Dec 8;28(24):8014. doi: 10.3390/molecules28248014.
2
An evaluation of fusion partner proteins for paratransgenesis in Asaia bogorensis.
PLoS One. 2022 Sep 1;17(9):e0273568. doi: 10.1371/journal.pone.0273568. eCollection 2022.
3
Ecdysteroid kinase-like (EcKL) paralogs confer developmental tolerance to caffeine in .
Curr Res Insect Sci. 2022 Jan 16;2:100030. doi: 10.1016/j.cris.2022.100030. eCollection 2022.
4
Novel Signal Sequences for Inhibition in .
Front Microbiol. 2021 Feb 16;12:633667. doi: 10.3389/fmicb.2021.633667. eCollection 2021.
5
Improving the Expression of Human Granulocyte Colony Stimulating Factor in Escherichia coli by Reducing the GC-content and Increasing mRNA Folding Free Energy at 5'-Terminal End.
Adv Pharm Bull. 2020 Sep;10(4):610-616. doi: 10.34172/apb.2020.073. Epub 2020 Aug 9.
6
Methylase-assisted subcloning for high throughput BioBrick assembly.
PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717/peerj.9841. eCollection 2020.
7
Construction of a Pichia pastoris strain efficiently producing recombinant human granulocyte-colony stimulating factor (rhG-CSF) and study of its biological activity on bone marrow cells.
Mol Biol Rep. 2020 Jan;47(1):607-620. doi: 10.1007/s11033-019-05169-9. Epub 2019 Nov 11.
8
Pyrite cloning: a single tube and programmed reaction cloning with restriction enzymes.
Plant Methods. 2018 Oct 17;14:91. doi: 10.1186/s13007-018-0359-7. eCollection 2018.
9
Blood meal-induced inhibition of vector-borne disease by transgenic microbiota.
Nat Commun. 2018 Oct 8;9(1):4127. doi: 10.1038/s41467-018-06580-9.
10
Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field.
Biochem Biophys Rep. 2016 Oct 15;8:360-364. doi: 10.1016/j.bbrep.2016.10.006. eCollection 2016 Dec.
本文引用的文献
1
Hexamine cobalt chloride promotes intermolecular ligation of blunt end DNA fragments by T4 DNA ligase.
Nucleic Acids Res. 1985 Mar 25;13(6):1997-2008. doi: 10.1093/nar/13.6.1997.
2
Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors.
Biotechniques. 1992 Oct;13(4):515-8.
Zotero 插件