Peltier J B, Rossignol M
Biochimie et Physiologie Végétales, INRA/ENSA-M/CNRS URA 573, Montpellier, France.
Biochem Biophys Res Commun. 1996 Feb 15;219(2):492-6. doi: 10.1006/bbrc.1996.0261.
Plasma membrane vesicles form suspension cultured tobacco cells were fractionated by counter-current phase partitioning. The subfractions exhibited identical biochemical characteristics and differed mainly by their sidedness. In addition, fluorescence polarization experiments indicated slight fluidity or order differences. These characteristics remained unchanged when cells were treated with auxin, prior to membrane purification. The sensitivity to auxin of the H+-ATPase catalysed proton translocation was investigated in plasma membrane subfractions. Identical sensitivity was measured in all subfractions from control cells. After auxin treatment of cells, a dramatic increase in the sensitivity was observed in one subfraction. Results are discussed in relation to the recent report of an auxin-induced clustering of the putative auxin-receptor (Diekmann et al., Proc. Natl. Acd. Sci USA 92, 3425-3429, 1995).
通过逆流相分配对悬浮培养的烟草细胞形成的质膜囊泡进行分级分离。这些亚级分表现出相同的生化特性，主要区别在于它们的内外侧性。此外，荧光偏振实验表明存在轻微的流动性或有序性差异。在用生长素处理细胞后进行膜纯化时，这些特性保持不变。在质膜亚级分中研究了H + -ATP酶催化的质子转运对生长素的敏感性。在对照细胞的所有亚级分中测得相同的敏感性。用生长素处理细胞后，在一个亚级分中观察到敏感性急剧增加。结合最近关于生长素诱导假定生长素受体聚集的报道（Diekmann等人，《美国国家科学院院刊》92，3425 - 3429，1995）对结果进行了讨论。
