Santoni V, Vansuyt G, Rossignol M
Lab. Biochimie et Physiologie Végétales, INRA/ENSA-M/CNRS UA 573, Montpellier, France.
FEBS Lett. 1993 Jul 12;326(1-3):17-20. doi: 10.1016/0014-5793(93)81751-k.
The sensitivity to auxin of the H(+)-ATPase-mediated proton translocation was investigated in vitro using purified plasma membrane vesicles from tobacco leaves. In vivo pretreatment of tobacco plants with auxin promotes a 100-fold increase of the in vitro sensitivity to auxin. This effect is specific for biologically active auxins and is dose- and time-dependent. In addition, pretreatment with auxin induces the accumulation of several polypeptides in the plasma membrane. These polypeptides constitute the first set of hormone-responsive polypeptides evidenced in plant membranes.
利用从烟草叶片中纯化得到的质膜囊泡在体外研究了H(+)-ATP酶介导的质子转运对生长素的敏感性。用生长素对烟草植株进行体内预处理可使体外对生长素的敏感性提高100倍。这种效应对于具有生物活性的生长素是特异的，并且呈剂量和时间依赖性。此外，用生长素预处理可诱导质膜中几种多肽的积累。这些多肽构成了在植物膜中证实的第一组激素反应性多肽。
