Malagié I, Trillat A C, Jacquot C, Gardier A M
Faculté de Pharmacie, Laboratorie de Pharmacologie JE 92-372, Chatenay-Malabry, France.
Eur J Pharmacol. 1995 Nov 14;286(2):213-7. doi: 10.1016/0014-2999(95)00573-4.
Acute administration of fluoxetine (1, 10 and 20 mg/kg i.p.) increased extracellular levels of serotonin (5-hydroxytryptamine, 5-HT) in the frontal cortex, ventral hippocampus and raphe nuclei as measured by in vivo microdialysis in anaesthetized rats. In the frontal cortex, fluoxetine showed a marked dose-response effect whereas in the ventral hippocampus and raphe nuclei the fluoxetine-induced effect was maximum at 10 mg/kg. However, the maximal increase in 5-HT was observed in the cell body-containing area, the raphe nuclei. The order of changes in extracellular 5-HT was raphe nuclei > ventral hippocampus > frontal cortex. Our results add further arguments in favour of the key role played by raphe nuclei in the mechanism of action of serotoninergic antidepressant drugs.
通过对麻醉大鼠进行体内微透析测量发现，急性给予氟西汀（腹腔注射，剂量为1、10和20毫克/千克）可提高额叶皮质、腹侧海马体和中缝核中血清素（5-羟色胺，5-HT）的细胞外水平。在额叶皮质中，氟西汀呈现出明显的剂量反应效应，而在腹侧海马体和中缝核中，氟西汀诱导的效应在10毫克/千克时最大。然而，5-HT的最大增加出现在含有细胞体的区域，即中缝核。细胞外5-HT变化的顺序为中缝核>腹侧海马体>额叶皮质。我们的结果进一步支持了中缝核在血清素能抗抑郁药物作用机制中所起的关键作用。
