Gómez de Segura I A, Aguilera M J, Codesal J, Codoceo R, De-Miguel E
Servicio de Cirugía Experimental, Hospital "La Paz", Madrid, Spain.
J Surg Res. 1996 Apr;62(1):5-10. doi: 10.1006/jsre.1996.0164.
Our aim was to assess the proliferative effect of human growth hormone on ileal mucosa after two different adaptation models of massive small and massive large bowel resection. Male Wistar rats were assigned to control-laparotomy, 90% small bowel resection, or 75% large bowel resection and were treated with either saline or human growth hormone daily for 7 days (total six groups; n = 8/group). Ileal proliferative status was assessed by means of histomorphometry and proliferating cell nuclear antigen. Plasma somatostatin was quantitated. Growth hormone increased (P < 0.01) mucosal height in all groups with a more marked effect on the crypt than on villus height. Proliferating cell nuclear antigen-labeled cells increased similarly (P < 0.01). Small bowel resection appears to favor a more marked increment in villus height than large bowel resection. Compared to control saline-treated group, the remaining groups showed decreases in plasma somatostatin (P < 0.01). Human growth hormone has a marked trophic effect on intestinal mucosa, even in hyperproliferative states. Decreased plasma somatostatin associated with intestinal hyperplastic mucosa suggests a possible relationship with the adaptive process.
我们的目的是评估在两种不同的大量小肠和大量大肠切除适应模型后，人生长激素对回肠黏膜的增殖作用。将雄性Wistar大鼠分为假手术对照组、90%小肠切除组或75%大肠切除组，并每天给予生理盐水或人生长激素治疗7天（共六组；每组n = 8）。通过组织形态计量学和增殖细胞核抗原评估回肠增殖状态。对血浆生长抑素进行定量分析。生长激素使所有组的黏膜高度增加（P < 0.01），对隐窝的影响比对绒毛高度的影响更显著。增殖细胞核抗原标记的细胞也同样增加（P < 0.01）。小肠切除似乎比大肠切除更有利于绒毛高度的显著增加。与生理盐水治疗的对照组相比，其余各组血浆生长抑素水平降低（P < 0.01）。人生长激素即使在高增殖状态下对肠黏膜也有显著的营养作用。与肠道增生性黏膜相关的血浆生长抑素降低提示其与适应过程可能存在关联。
