El Maradny E, Kanayama N, Halim A, Maehara K, Kobayashi T, Terao T
Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Japan.
Acta Obstet Gynecol Scand. 1996 Mar;75(3):203-7. doi: 10.3109/00016349609047087.
This research was aimed to study the biochemical changes in cervical mucus after application of laminaria tent.
Cervical mucus was collected before and after insertion of laminaria tent from 20 normal pregnant women in the first and second trimesters. Interleukin-1 beta, IL-8, collagenase activity, elastase activity, PGE2 and PGF2 alpha were measured in the collected samples. Laminaria tent was also inserted in one vaginal cervix of five non-pregnant rabbits and the clinical and histological changes were compared with five non-treated rabbits. Collagen contents were assessed histologically and Leukocyte number was counted.
IL-1 beta and IL-8 and elastase activity were significantly increased after insertion of laminaria (p<0.0001 and 0.001 respectively). Collagenase activity was not significantly changed. Whereas concentration of PGE2 and PGF2 alpha in the mucus were markedly increased (p<0.0001 and 0.001 respectively). Collagen contents were significantly decreased in laminaria and opposite side cervices (p<0.0001). No significant changes in leukocyte count could be observed in treated and non treated cervices.
Insertion of laminaria leads to increase concentration of IL-1 beta, IL-8, PGE2, PGF2 alpha and elastase activity. Mechanical stretching of the cervix and biochemical changes in cervical mucus may explain the mechanism of cervical ripening induced by laminaria.
本研究旨在探讨海藻棒应用后宫颈黏液的生化变化。
收集20例孕早期及孕中期正常孕妇放置海藻棒前后的宫颈黏液。检测所收集样本中的白细胞介素-1β、白细胞介素-8、胶原酶活性、弹性蛋白酶活性、前列腺素E2和前列腺素F2α。将海藻棒插入5只未孕兔的一侧阴道宫颈，并与5只未处理的兔比较临床及组织学变化。组织学评估胶原含量并计数白细胞数量。
放置海藻棒后白细胞介素-1β、白细胞介素-8及弹性蛋白酶活性显著升高（分别为p<0.0001和0.001）。胶原酶活性无显著变化。而黏液中前列腺素E2和前列腺素F2α的浓度显著升高（分别为p<0.0001和0.001）。海藻棒及对侧宫颈的胶原含量显著降低（p<0.0001）。处理组和未处理组宫颈的白细胞计数无显著变化。
放置海藻棒导致白细胞介素-1β、白细胞介素-8、前列腺素E2、前列腺素F2α浓度及弹性蛋白酶活性升高。宫颈的机械性扩张及宫颈黏液的生化变化可能解释海藻棒诱导宫颈成熟的机制。
