Ingemansson R, Sjöberg T, Steen S
Department of Cardiothoracic Surgery, University Hospital of Lund, Sweden.
Ann Thorac Surg. 1996 Apr;61(4):1158-62. doi: 10.1016/0003-4975(95)01141-2.
The aim was to investigate the effect of calcium in organ preservation solutions with respect to 36-hour preservation of vascular smooth muscle function and endothelium-dependent relaxation.
The infrarenal aortas of 60 Sprague-Dawley rats were studied in organ baths as fresh controls and after 36 hours of cold (4 degrees C) storage in different preservation solutions with and without calcium. The thromboxane A2 analogue U-46619 was used to study contractility. Endothelium-dependent relaxation was tested by the cumulative addition of acetylcholine. Papaverine hydrochloride was used to elicit endothelium-independent relaxation.
Krebs solution was the only solution able to fully preserve contractility. Krebs solution without calcium gave poor preservation. After the addition of 1.5 mmol/L of calcium to University of Wisconsin solution and to Perfadex, both these solutions became fully able to preserve contractility. None of the solutions (with or without calcium) were fully able to preserve endothelium-dependent relaxation, although University of Wisconsin solution gave good preservation and Perfadex, fair preservation. Euro-Collins solution and K+ (124 mmol/L)-enriched Krebs solution were not able to preserve smooth muscle function or endothelium-dependent relaxation.
Calcium is essential for long-term preservation of vascular smooth muscle function but not for long-term preservation of endothelium-dependent relaxation.
目的是研究器官保存液中的钙对血管平滑肌功能和内皮依赖性舒张功能36小时保存的影响。
对60只Sprague-Dawley大鼠的肾下腹主动脉进行研究，分别作为新鲜对照以及在不同含钙或不含钙的保存液中于4℃冷藏36小时后进行研究。使用血栓素A2类似物U-46619研究收缩性。通过累积添加乙酰胆碱来测试内皮依赖性舒张功能。使用盐酸罂粟碱引发非内皮依赖性舒张功能。
克雷布斯溶液是唯一能够完全保存收缩性的溶液。不含钙的克雷布斯溶液保存效果不佳。向威斯康星大学溶液和Perfadex溶液中添加1.5 mmol/L的钙后，这两种溶液均能够完全保存收缩性。尽管威斯康星大学溶液保存效果良好，Perfadex溶液保存效果尚可，但没有一种溶液（含钙或不含钙）能够完全保存内皮依赖性舒张功能。欧洲柯林斯溶液和富含钾离子（124 mmol/L）的克雷布斯溶液无法保存平滑肌功能或内皮依赖性舒张功能。
钙对于血管平滑肌功能的长期保存至关重要，但对于内皮依赖性舒张功能的长期保存并非必需。
