Ingemansson R, Massa G, Pandita R K, Sjöberg T, Steen S
Department of Cardiothoracic Surgery, University Hospital, Lund, Sweden.
Ann Thorac Surg. 1995 Nov;60(5):1210-4. doi: 10.1016/0003-4975(95)00548-Y.
The aim of this study was to compare Perfadex with Euro-Collins solution regarding 24-hour preservation of endothelium-dependent relaxation and vascular smooth muscle function.
The infrarenal aorta of 72 isogenic rats was studied in organ baths as fresh controls, after 24 hours of cold (4 degrees C) storage, and after 24-hour storage followed by transplantation and examination after 7 or 30 days. The thromboxane A2 analogue U-46619 was used to test contractility. Acetylcholine chloride was used to elicit endothelium-dependent relaxation and papaverine hydrochloride, to elicit endothelium-independent relaxation.
With both solutions, all grafts were patent after 7 and 30 days. Vessels preserved in Euro-Collins solution for 24 hours lost 95% (p < 0.001) of their contractility compared with fresh controls; 7 days after transplantation, they had regained 40% of initial contractility, and after 30 days, there was no significant decrease in contractility. Vessels preserved in Perfadex manifested no significant decrease in contractility at any time. Endothelium-dependent relaxation could not be evaluated in vessels stored for 24 hours in Euro-Collins solution because they had lost almost all contractility; 7 days after transplantation, endothelium-dependent relaxation was reduced by 65% (p < 0.001), but at 30 days after transplantation, there was no significant decrease in endothelium-dependent relaxation. Vessels preserved in Perfadex for 24 hours lost 17% (p < 0.05) of endothelium-dependent relaxation, but 7 and 30 days after transplantation, there was no significant decrease in endothelium-dependent relaxation.
Perfadex, but not Euro-Collins solution, has the capacity to preserve vascular function after 24 hours of storage followed by in vivo reperfusion.
本研究旨在比较Perfadex溶液与Euro - Collins溶液在保存内皮依赖性舒张和血管平滑肌功能24小时方面的效果。
对72只同基因大鼠的肾下腹主动脉进行研究，分别作为新鲜对照、在4℃冷藏24小时后、以及在24小时保存后进行移植并于7天或30天后检查，均在器官浴中进行。使用血栓素A2类似物U - 46619测试收缩性。使用氯化乙酰胆碱引发内皮依赖性舒张，使用盐酸罂粟碱引发非内皮依赖性舒张。
两种溶液处理的移植物在7天和30天后均保持通畅。与新鲜对照相比，在Euro - Collins溶液中保存24小时的血管失去了95%（p < 0.001）的收缩性；移植7天后，它们恢复了初始收缩性的40%，30天后，收缩性无显著下降。保存在Perfadex溶液中的血管在任何时候收缩性均无显著下降。在Euro - Collins溶液中保存24小时的血管无法评估内皮依赖性舒张，因为它们几乎失去了所有收缩性；移植7天后，内皮依赖性舒张降低了65%（p < 0.001），但在移植30天后，内皮依赖性舒张无显著下降。保存在Perfadex溶液中24小时的血管内皮依赖性舒张降低了17%（p < 0.05），但在移植7天和30天后，内皮依赖性舒张无显著下降。
Perfadex溶液而非Euro - Collins溶液能够在24小时保存后经体内再灌注仍保持血管功能。
