Nakazato M, Yamaguchi H, Kinoshita H, Kangawa K, Matsuo H, Chino N, Matsukura S
Third Department of Internal Medicine, Miyazaki Medical College, Japan.
Biochem Biophys Res Commun. 1996 Mar 27;220(3):586-93. doi: 10.1006/bbrc.1996.0447.
Uroguanylin, a 16-amino acid peptide, is an endogenous activator of intestinal and possibly renal guanylate cyclase C (GC-C). Using two synthetic topological isoforms of human uroguanylin, one bioactive, the other inactive, we prepared two antisera specific for the individual isoforms and developed sensitive radioimmunoassay (RIAs). The respective plasma concentrations of the bioactive and inactive uroguanylins in the normal individuals tested were 5.0 +/- 0.3 fmol/ml (mean +/- SE) and 1.6 +/- 0.1 fmol/ml. These concentrations increased in chronic renal failure (CRF). The major endogenous uroguanylin molecule in normal human urine was 16 amino acids long, whereas in the plasma and urine of CRF patients the major molecule was 10-kDa prouroguanylin. The RlAs established are promising tools for clarifying the physiological functions and pathophysiological implications of uroguanylin in water and electrolyte homeostasis.
尿鸟苷素是一种由16个氨基酸组成的肽，是肠及可能的肾鸟苷酸环化酶C（GC - C）的内源性激活剂。我们使用两种合成的人尿鸟苷素拓扑异构体，一种具有生物活性，另一种无活性，制备了针对各异构体的两种抗血清，并开发了灵敏的放射免疫分析法（RIA）。在受试的正常个体中，生物活性尿鸟苷素和无活性尿鸟苷素各自的血浆浓度分别为5.0±0.3 fmol/ml（平均值±标准误）和1.6±0.1 fmol/ml。这些浓度在慢性肾衰竭（CRF）时升高。正常人尿液中主要的内源性尿鸟苷素分子由16个氨基酸组成，而在CRF患者的血浆和尿液中，主要分子是10 kDa的尿鸟苷素原。所建立的放射免疫分析法是阐明尿鸟苷素在水和电解质稳态中的生理功能及病理生理意义的有前景的工具。
