Burcham P C, Kuhan Y T
Department of Clinical and Experimental Pharmacology, The University of Adelaide, Australia.
Biochem Biophys Res Commun. 1996 Mar 27;220(3):996-1001. doi: 10.1006/bbrc.1996.0521.
Incubation of model proteins with the toxic lipid peroxidation product malondialdehyde resulted in a time- and concentration-dependent increase in carbonyl contents. Carbonyl groups were detected either spectrophotometrically or immunochemically after derivatization with 2,4-dinitrophenylhydrazine. Although significant adduction occurred when modifications were performed at pH 7.0, carbonyl formation was most extensive when modifications were carried out at pH 4.0 or pH 5.0. Similarly, formation of intermolecular crosslinks was most extensive when reactions were carried out under mildly acidic conditions. Our results raise the possibility that malondialdehyde adducts contribute to the carbonyl content of proteins recovered from mammalian tissues.
将模型蛋白与有毒的脂质过氧化产物丙二醛一起孵育，会导致羰基含量随时间和浓度增加。在用2,4-二硝基苯肼衍生化后，通过分光光度法或免疫化学法检测羰基。尽管在pH 7.0进行修饰时会发生显著的加合反应，但在pH 4.0或pH 5.0进行修饰时，羰基形成最为广泛。同样，在轻度酸性条件下进行反应时，分子间交联的形成最为广泛。我们的结果增加了丙二醛加合物导致从哺乳动物组织中回收的蛋白质羰基含量增加的可能性。
