Chakraborty A, Chakraborty N G, Chattopadhyay U
Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Calcutta, India.
Int J Cancer. 1996 Mar 28;66(1):65-9. doi: 10.1002/(SICI)1097-0215(19960328)66:1<65::AID-IJC12>3.0.CO;2-A.
The regulatory role of prolactin (Prl) on peripheral blood natural killer (NK) and lymphokine-activated killer (LAK) cell activities was studied in young (mean age, 40 years) and elderly (mean age, 68 years) healthy men and patients with carcinoma of the oral cavity (oral cancer). The peripheral blood NK cells, but not the LAK cells, were found to be depressed in oral cancer patients compared with age-matched healthy men. However, age-associated deficiency in both NK and LAK cell activity was observed in healthy men and cancer patients. Prl produced dose-dependent inhibition (1, 10, 100 or 250 ng/ml) or stimulation (25-50 ng/ml) of resting NK cells in young groups of healthy men and cancer patients. In elderly groups less or no response of the NK cells to low doses of Prl (1-10 ng/ml) was evident. The NK cells of young and elderly healthy men were stimulated by human recombinant Interleukin-2 (rIL-2) (100 U/ml), and Prl (1-25O ng/ml) inhibited these cells. In oral cancer patients an altered response to low doses of Prl (1-5O ng/ ml) was observed in IL-2-stimulated NK cells, which also revealed malignancy- associated loss of IL-2 response. In contrast, there was no malignancy or age-associated change in Prl response of the LAK cells. Treatment of peripheral blood lymphocytes of both healthy men and oral cancer patients for 5 days with Prl (50 ng/ml) in the ++presence of low concentration of serum generated LAK cells.
在年轻（平均年龄40岁）和老年（平均年龄68岁）的健康男性以及口腔癌患者中，研究了催乳素（Prl）对外周血自然杀伤（NK）细胞和淋巴因子激活的杀伤（LAK）细胞活性的调节作用。与年龄匹配的健康男性相比，口腔癌患者外周血中的NK细胞活性降低，但LAK细胞活性未降低。然而，在健康男性和癌症患者中均观察到与年龄相关的NK和LAK细胞活性缺乏。在年轻的健康男性和癌症患者组中，Prl对静息NK细胞产生剂量依赖性抑制（1、10、100或250 ng/ml）或刺激（25 - 50 ng/ml）。在老年组中，NK细胞对低剂量Prl（1 - 10 ng/ml）的反应较弱或无明显反应。年轻和老年健康男性的NK细胞受到重组人白细胞介素-2（rIL-2）（100 U/ml）刺激，而Prl（1 - 250 ng/ml）抑制这些细胞。在口腔癌患者中，观察到IL-2刺激的NK细胞对低剂量Prl（1 - 50 ng/ml）的反应改变，这也显示出与恶性肿瘤相关的IL-2反应丧失。相比之下，LAK细胞的Prl反应没有与恶性肿瘤或年龄相关的变化。在低浓度血清存在的情况下，用Prl（50 ng/ml）处理健康男性和口腔癌患者的外周血淋巴细胞5天可产生LAK细胞。
