Inoue T, Hasumi K, Kuniyasu T, Endo A
Department of Applied Biological Science, Tokyo Noko University, Japan.
J Antibiot (Tokyo). 1996 Jan;49(1):45-9. doi: 10.7164/antibiotics.49.45.
Four novel cyclic pentapeptides, designated plactins A, B, C and D, were isolated by solvent extraction and reverse-phase HPLC from mycelium of a fungal strain F165 that belongs to the order of Agonomycetales. By a combination of chemical and spectroscopic analyses and chemical synthesis, the structures of plactins A, B, C and D were determined to be cyclo(-D-Val-L-Leu-D-alloIle-L-Try-D-Arg-), cyclo(-D-Val-L-Leu-D-Leu-L-Tyr-D-Arg-), cyclo(-D-Val-L-Leu-D-alloIle-L-Phe-D-Arg-) and cyclo(-D-Val-L-Leu-D-Leu-L-Phe-D-Arg-), respectively. Plactins stimulated U937 cell-mediated degradation of 125I-fibrin plates by 50% at a concentration of 7.5 approximately 32 microM.
通过溶剂萃取和反相高效液相色谱法，从属于无柄盘菌目的真菌菌株F165的菌丝体中分离出四种新型环五肽，分别命名为普拉汀A、B、C和D。通过化学分析、光谱分析和化学合成相结合的方法，确定普拉汀A、B、C和D的结构分别为环（-D-缬氨酸-L-亮氨酸-D-别异亮氨酸-L-色氨酸-D-精氨酸-）、环（-D-缬氨酸-L-亮氨酸-D-亮氨酸-L-酪氨酸-D-精氨酸-）、环（-D-缬氨酸-L-亮氨酸-D-别异亮氨酸-L-苯丙氨酸-D-精氨酸-）和环（-D-缬氨酸-L-亮氨酸-D-亮氨酸-L-苯丙氨酸-D-精氨酸-）。普拉汀在浓度约为7.5至32微摩尔时，可使U937细胞介导的125I-纤维蛋白平板降解率提高50%。
