Arduini A, Dottori S, Sciarroni A F, Corsico N, Morabito E, Arrigoni-Martelli E, Calvani M
Institute of Biochemical Sciences, University of G. D'Annunzio, Chieti, Italy.
Mol Cell Biochem. 1995 Nov 8;152(1):31-7. doi: 10.1007/BF01076461.
In this work we have examined the effect of the oral administration of propionyl-L-carnitine (PLC) on the membrane phospholipid fatty acid turnover of erythrocytes from streptozotocin-induced diabetic rats. A statistically significant reduction in radioactive palmitate, oleate, and linoleate, but not arachidonate, incorporation into membrane phosphatidylcholine (PC) of diabetic rat erythrocytes with respect to control animals was found. Changes in radioactive fatty acid incorporation were also found in diabetic red cell phosphatidylethanolamine (PE), though they were not statistically significant. Oral propionyl-L-carnitine (PLC) treatment of diabetic rats partially restored the ability of intact red cells to reacylate membrane PC with palmitate and oleate, and reacylation with linoleate was fully restored. The analysis of the membrane phospholipid fatty acid composition revealed a consistent increase of linoleate levels in diabetic rat red cells, a modest decrease of palmitate, oleate and arachidonate. The phospholipid fatty acid composition of diabetic red blood cells was not affected by the PLC treatment. Lysophosphatidylcholine acyl-CoA transferase (LAT) specific activity measured with either palmitoyl-CoA or oleyl-CoA was significantly reduced in diabetic erythrocyte membranes in comparison to controls. In addition, LAT kinetic parameters of diabetic erythrocytes were altered. The reduced LAT activity could be partially corrected by PLC treatment of diabetic rats. Our data suggest that the impaired erythrocyte membrane physiological expression induced by the diabetic disease may be attenuated by the beneficial activity of PLC on the red cell membrane phospholipid fatty acid turnover.
在本研究中，我们检测了口服丙酰-L-肉碱（PLC）对链脲佐菌素诱导的糖尿病大鼠红细胞膜磷脂脂肪酸周转的影响。与对照动物相比，我们发现糖尿病大鼠红细胞膜磷脂酰胆碱（PC）中放射性棕榈酸、油酸和亚油酸（而非花生四烯酸）掺入量有统计学意义的降低。在糖尿病红细胞磷脂酰乙醇胺（PE）中也发现了放射性脂肪酸掺入的变化，尽管无统计学意义。对糖尿病大鼠进行口服丙酰-L-肉碱（PLC）治疗后，完整红细胞用棕榈酸和油酸重新酰化膜PC的能力部分恢复，用亚油酸重新酰化的能力完全恢复。膜磷脂脂肪酸组成分析显示，糖尿病大鼠红细胞中亚油酸水平持续升高，棕榈酸、油酸和花生四烯酸略有降低。糖尿病红细胞的磷脂脂肪酸组成不受PLC治疗的影响。与对照组相比，糖尿病红细胞膜中用棕榈酰辅酶A或油酰辅酶A测定的溶血磷脂酰胆碱酰基辅酶A转移酶（LAT）比活性显著降低。此外，糖尿病红细胞的LAT动力学参数发生改变。糖尿病大鼠经PLC治疗后，LAT活性降低可得到部分纠正。我们的数据表明，糖尿病引起的红细胞膜生理表达受损可能因PLC对红细胞膜磷脂脂肪酸周转的有益作用而减轻。
