Akari H, Mori K, Terao K, Otani I, Fukasawa M, Mukai R, Yoshikawa Y
Tsukuba Primate Center for Medical Science, National Institute of Health, Ibaraki, Japan.
Virology. 1996 Apr 15;218(2):382-8. doi: 10.1006/viro.1996.0207.
Peripheral blood T lymphocytes of Old World monkeys, rhesus and cynomolgus monkey (Macaca mulatta and Macaca fascicularis, respectively), were successfully immortalized by infection with Herpesvirus saimiri subtype C. The T cell lines were stably cultured without addition of exogenous IL-2. The STP-C488 protein, the oncogene product of subtype C strain 488-77, was detected in these cells by Western blotting. They also expressed some markers of activated or matured T cell phenotypes such as CD2+, monkey Pan-T+, CD25+,CD29+ and MHC-II DR+. Interestingly, not only CD4+CD8- or CD4-CD8+ single positive subpopulations but also CD4+CD8+ double positive ones were present in all of them. Furthermore, they were productively infected with both SIVmac and SIVagm. The levels of the viral replication were comparable to those in human T cell lines. Thus, Herpes Virus Saimiri-immortalized Old World monkey T lymphocytes will be suitable for further studies of immune system in Old World monkeys and cell-virus interactions in SIV infection.
通过感染C亚型赛米利疱疹病毒，成功使旧世界猴（恒河猴和食蟹猴，分别为猕猴属和食蟹猕猴）的外周血T淋巴细胞永生化。在不添加外源性白细胞介素-2的情况下，T细胞系得以稳定培养。通过蛋白质免疫印迹法在这些细胞中检测到了C亚型毒株488 - 77的癌基因产物STP - C488蛋白。它们还表达了一些活化或成熟T细胞表型的标志物，如CD2 +、猴泛T +、CD25 +、CD29 +和MHC - II DR +。有趣的是，所有这些细胞中不仅存在CD4 + CD8 -或CD4 - CD8 +单阳性亚群，还存在CD4 + CD8 +双阳性亚群。此外，它们被猴免疫缺陷病毒（SIVmac）和非洲绿猴免疫缺陷病毒（SIVagm）有效感染。病毒复制水平与人类T细胞系中的相当。因此，赛米利疱疹病毒永生化的旧世界猴T淋巴细胞将适用于进一步研究旧世界猴的免疫系统以及SIV感染中的细胞 - 病毒相互作用。
