Schmitz B, Park I A, Kaufmann R, Thiele J, Fischer R
Institute of Pathology, University of Cologne, Germany.
Acta Haematol. 1995;94(4):173-81. doi: 10.1159/000204006.
Interactions between stromal cells or extracellular matrix and hematopoietic cells are important factors for the very complex processes associated with differentiation and maturation in the bone marrow. To elucidate these multifold processes, the expression pattern of various adhesion molecules was studied on enriched fibroblastic populations derived from healthy volunteers. CD44 (homing cell adhesion molecule) and very late activation antigen beta 1 (VLA beta 1; CD29) could be demonstrated on almost all fibroblasts without an alteration following cytokine stimulation. On the other hand, VLA-2 (CDw49b), VLA-3 (CDw49c), VLA-4 (CDw49d), VLA-5 (CDw49e), intercellular adhesion molecule-1 (ICAM-1; CD54) and vascular cell adhesion molecule-1 (VCAM-1; CD106) were only represented by certain cell fractions. In our studies recombinant human granulocyte macrophage-colony stimulating factor failed to alter the expression pattern of these adhesion molecules, whereas recombinant human interleukin-3 (rhIL-3 showed a tendency for downregulation of the VLA antigens except for VLA beta 1. However, recombinant human transforming growth factor-beta 1 (rhTGF beta 1) exerted a reducing effect on the expression of VLA-3 and induced an increase in the VLA-5-positive fraction. In the immunoglobin class VCAM-1 revealed a decrease staining capacity after stimulation with rhTGF beta 1 and rhIL-3. Contrary to this finding, the presentation of ICAM-1 increased after administration of these mediators.
基质细胞或细胞外基质与造血细胞之间的相互作用是骨髓中与分化和成熟相关的非常复杂过程的重要因素。为了阐明这些多方面的过程，我们研究了来自健康志愿者的富集成纤维细胞群体上各种黏附分子的表达模式。几乎所有成纤维细胞上都能检测到CD44（归巢细胞黏附分子）和极晚期活化抗原β1（VLAβ1；CD29），细胞因子刺激后其表达无变化。另一方面，VLA - 2（CDw49b）、VLA - 3（CDw49c）、VLA - 4（CDw49d）、VLA - 5（CDw49e）、细胞间黏附分子 - 1（ICAM - 1；CD54）和血管细胞黏附分子 - 1（VCAM - 1；CD106）仅在某些细胞组分中表达。在我们的研究中，重组人粒细胞巨噬细胞集落刺激因子未能改变这些黏附分子的表达模式，而重组人白细胞介素 - 3（rhIL - 3）除VLAβ1外，对VLA抗原表现出下调趋势。然而，重组人转化生长因子 - β1（rhTGFβ1）对VLA - 3的表达有降低作用，并诱导VLA - 5阳性组分增加。在免疫球蛋白类别中，VCAM - 1在rhTGFβ1和rhIL - 3刺激后染色能力下降。与这一发现相反，这些介质给药后ICAM - 1的表达增加。
