营养性铜缺乏相关性中性粒细胞减少症患者抗中性粒细胞抗体的特征分析

Characterization of antineutrophil antibodies in patients with neutropenia associated with nutritional copper deficiency.

作者信息

Higuchi S, Hirashima M, Nunoi H, Higashi A, Naoe H, Matsuda I

机构信息

Department of Pediatrics, National Saishunso Hospital, Kumamoto, Japan.

出版信息

Acta Haematol. 1995;94(4):192-5. doi: 10.1159/000204008.

DOI:10.1159/000204008

PMID:8610476

Abstract

Six antineutrophil antibody (ANA)-positive patients with copper deficiency were classified into two groups; those with (group A, n = 3) and those without (group B, n = 3) neutropenia. The percent binding of ANA for normal peripheral neutrophils was similar in both groups (83.5 +/- 7.2 vs. 79.1 +/- 10.5%). The percent binding of sera to cultured promyelocytic leukemia cells (HL-60) was increased in group A (from 3.7 +/- 3.2 to 12.2 +/- 2.3%) but not in group B (from 65.3 +/- 21.7 to 40.7 +/- 6.3%) after stimulation of HL-60 with DMSO. The stimulated HL-60 cells expressed CD 16 and CD 11b antigens. In the presence of monoclonal antibody for CD 16, the titer of ANA was nil in group A and unchanged in group B. Thus, ANA of patients with neutropenia may recognize mainly the CD 16 antigen, the Fc gamma receptor III of neutrophils.

摘要

6例抗中性粒细胞抗体（ANA）阳性的铜缺乏患者被分为两组：有中性粒细胞减少症的患者（A组，n = 3）和无中性粒细胞减少症的患者（B组，n = 3）。两组中ANA与正常外周中性粒细胞的结合百分比相似（83.5±7.2对79.1±10.5%）。用二甲基亚砜（DMSO）刺激人早幼粒白血病细胞（HL-60）后，A组血清与培养的HL-60细胞的结合百分比增加（从3.7±3.2%增至12.2±2.3%），而B组未增加（从65.3±21.7%降至40.7±6.3%）。受刺激的HL-60细胞表达CD16和CD11b抗原。在存在抗CD16单克隆抗体的情况下，A组ANA滴度为零，B组不变。因此，中性粒细胞减少症患者的ANA可能主要识别CD16抗原，即中性粒细胞的Fcγ受体III。