Murthy V V, Gilbert J C, Goldberg L I, Kuo J F
J Pharm Pharmacol. 1976 Jul;28(7):567-71. doi: 10.1111/j.2042-7158.1976.tb02797.x.
To characterize further a putative dopamine receptor in the renal artery, the effects of dopamine on canine renal artery adenylate cyclase activity were studied. Since the femoral artery is thought to be devoid of a similar dopamine receptor, the effects of dopamine on the adenylate cyclase activity of the canine femoral artery were also studied. In tissues from dogs with or without phenoxybenzamine pretreatment, renal artery adenylate cyclase was maximally stimulated by 4 muM dopamine, compared to 20 muM required for the femoral artery enzyme. The concentrations of isoprenaline required to maximally stimulate renal and femoral artery adenylate cyclase were 0-04 and 0-2 muM, respectively. In tissue from the phenoxybenzamine-pretreated dog, the sitmulatroy effect of dopamine on the renal artery enzyme was selectively blocked by 0-01 muM haloperidol, but not by 0-2 muM propranolol. In the femoral artery, however, the dopamine stimulation was blocked by both antagonists. Stimulation by isoprenaline of renal and femoral artery adenylate cyclase was blocked by propranolol. These data suggest the concept that dopamine interacts with a specific artery receptor apparently different from alpha-and beta-adrenoceptors.
为了进一步表征肾动脉中一种假定的多巴胺受体，研究了多巴胺对犬肾动脉腺苷酸环化酶活性的影响。由于认为股动脉没有类似的多巴胺受体，因此也研究了多巴胺对犬股动脉腺苷酸环化酶活性的影响。在有或没有苯氧苄胺预处理的犬的组织中，与股动脉酶所需的20μM相比，4μM多巴胺可最大程度地刺激肾动脉腺苷酸环化酶。最大程度刺激肾和股动脉腺苷酸环化酶所需的异丙肾上腺素浓度分别为0.04和0.2μM。在苯氧苄胺预处理犬的组织中，0.01μM氟哌啶醇可选择性阻断多巴胺对肾动脉酶的刺激作用，但0.2μM普萘洛尔则不能。然而，在股动脉中，两种拮抗剂均可阻断多巴胺的刺激作用。普萘洛尔可阻断异丙肾上腺素对肾和股动脉腺苷酸环化酶的刺激作用。这些数据提示了这样一种概念，即多巴胺与一种明显不同于α和β肾上腺素能受体的特定动脉受体相互作用。
