Asou H, Eguchi M, Suzukawa K, Morishita K, Tanaka K, Date M, Hamamoto K, Kamada N
Department of Cancer Cytogenetics, Hiroshima University, Japan.
Br J Haematol. 1996 Apr;93(1):68-74. doi: 10.1046/j.1365-2141.1996.4821023.x.
A novel human leukaemia cell line (Kasumi-4) was established from the peripheral blood of a 6-year-old girl suffering from chronic myelogenous leukaemia (CML) in blast crisis. The Kasumi-4 cells had the following characteristic features: undifferentiated blasts which were positive from CD34, CD33 and CD13 surface markers, but negative for myeloperoxidase platelet peroxidase, CD36, CD41 and CD42; chromosome abnormalities of t(9;22;11) (q34;q11;q13), inv(3)(q21q26); and elevated expression of EVI1 gene which is located at chromosome band 3q26. Megakaryocytic maturation was not observed in the liquid culture following the addition of TPA, IL3, IL-6 or GM-CSF, b2-a2 type of BCR-ABL chimaeric messenger RNA was detected by RT-PCR analysis. This the first leukaemia cell line with a three-way translocation containing the the Ph chromosome and the second cell line with an inv(3)(q21q26). This cell line appears to be useful for studying the mechanisms of leukaemogenesis involving these chromosomal abnormalities and related oncogenes.
一株新的人类白血病细胞系（Kasumi-4）是从一名处于急变期的慢性粒细胞白血病（CML）6岁女孩的外周血中建立的。Kasumi-4细胞具有以下特征：未分化的原始细胞，其CD34、CD33和CD13表面标志物呈阳性，但髓过氧化物酶、血小板过氧化物酶、CD36、CD41和CD42呈阴性；染色体异常为t(9;22;11) (q34;q11;q13)、inv(3)(q21q26)；位于染色体3q26带的EVI1基因表达升高。添加TPA、IL3、IL-6或GM-CSF后，在液体培养中未观察到巨核细胞成熟，通过RT-PCR分析检测到b2-a2型BCR-ABL嵌合信使RNA。这是第一株含有费城染色体的具有三向易位的白血病细胞系，也是第二株具有inv(3)(q21q26)的细胞系。该细胞系似乎有助于研究涉及这些染色体异常和相关癌基因的白血病发生机制。
