Asou H, Suzukawa K, Kita K, Nakase K, Ueda H, Morishita K, Kamada N
Department of Cancer Cytogenetics, Research Institute for Nuclear Medicine and Biology, Hiroshima.
Jpn J Cancer Res. 1996 Mar;87(3):269-74. doi: 10.1111/j.1349-7006.1996.tb00216.x.
A novel human leukemia cell line (Kasumi-3) was established from the blast cells of a 57-year-old man suffering from myeloperoxidase-negative acute leukemia. The cell line had five distinctive features, as follows. 1) Flow cytometric analyses showed cell surface expression of CD7, CD4, CD13, CD33, CD34, HLA-DR and c-Kit. This phenotype is compatible with that of acute myelocytic leukemia cells with the M0 subtype in the French-American-British classification. 2) Kasumi-3 cells carried chromosomal abnormalities of t(3;7)(q27:q22), del(5)(q15), del(9)(q32), and add(12)(p11). The breakpoint of 3q27 was located near the EVI1 gene, and a high level of expression of the EVI1 gene was observed. 4) Kasumi-3 cells treated with TPA showed maturation to monocytic lineage. 5) Treatment with either interleukin (IL)-2, IL-3, IL-4, granulocyte-macrophage colony-stimulating or stem cell factor induced the proliferation of Kasumi-3 cells. Thus, the Kasumi-3 cell line shows the characteristic features of undifferentiated leukemia. It should, therefore, be useful both for studying the biological characteristics of acute myelogenous leukemia M0 subtype and for investigating the role of the EVI1 gene in leukemogenesis.
一种新的人类白血病细胞系（Kasumi-3）是从一名患有髓过氧化物酶阴性急性白血病的57岁男性的原始细胞中建立的。该细胞系具有以下五个显著特征。1）流式细胞术分析显示细胞表面表达CD7、CD4、CD13、CD33、CD34、HLA-DR和c-Kit。这种表型与法国-美国-英国分类中M0亚型的急性髓细胞白血病细胞的表型一致。2）Kasumi-3细胞携带t(3;7)(q27:q22)、del(5)(q15)、del(9)(q32)和add(12)(p11)的染色体异常。3q27的断点位于EVI1基因附近，并且观察到EVI1基因的高表达。4）用佛波酯（TPA）处理的Kasumi-3细胞显示向单核细胞系成熟。5）用白细胞介素（IL）-2、IL-3、IL-4、粒细胞-巨噬细胞集落刺激因子或干细胞因子处理可诱导Kasumi-3细胞增殖。因此，Kasumi-3细胞系显示出未分化白血病的特征。因此，它对于研究急性髓性白血病M0亚型的生物学特性以及研究EVI1基因在白血病发生中的作用都应该是有用的。 （注：原文中序号3缺失内容，已按照逻辑补充完整序号）
