羽毛降解地衣芽孢杆菌PWD-1角蛋白酶的产生与特性研究
Production and characterization of keratinase of a feather-degrading Bacillus licheniformis PWD-1.
作者信息
Cheng S W, Hu H M, Shen S W, Takagi H, Asano M, Tsai Y C
机构信息
Institute of Biochemistry, National Yang-Ming University, Taipei, Taiwan, Republic of China.
出版信息
Biosci Biotechnol Biochem. 1995 Dec;59(12):2239-43. doi: 10.1271/bbb.59.2239.
The keratinase produced by Bacillus licheniformis PWD-1 was induced by feather powder. Maximal enzyme production could be achieved by culturing in a medium containing 1% hammer-milled feather powder (100 mesh) at 45 degrees C for 30 h. Maximal growth of PWD-1 was achieved at 50 degrees C, and maximal enzyme induction was at 45 degrees C. The molecular mass and isoelectric point of this enzyme were 31.4 kDa and 8.5, respectively. This enzyme was stable from pH 5 to 12. The optimal reaction pHs for feather powder and casein were 8.5 and 10.5 to 11.5, respectively. The optimal reaction temperature was 50 degrees C to 55 degrees C. The relative activity of this enzyme toward casein, feather powder, keratin, elastin, and collagen was 100:52:41:18:7, and 100:56:32:3 for Suc-AAPL-pNA, Suc-AAPF-pNA, Suc-AAPM-pNA, and Suc-AAVA-pNA (Suc, succinyl; pNA, p-nitrophenylanilide).
地衣芽孢杆菌PWD-1产生的角蛋白酶由羽毛粉诱导产生。通过在含有1%锤磨羽毛粉（100目）的培养基中于45℃培养30小时可实现最大酶产量。PWD-1的最大生长温度为50℃，最大酶诱导温度为45℃。该酶的分子量和等电点分别为31.4 kDa和8.5。该酶在pH 5至12范围内稳定。羽毛粉和酪蛋白的最佳反应pH分别为8.5和10.5至11.5。最佳反应温度为50℃至55℃。该酶对酪蛋白、羽毛粉、角蛋白、弹性蛋白和胶原蛋白的相对活性为100:52:41:18:7，对琥珀酰-AAPL-pNA、琥珀酰-AAPF-pNA、琥珀酰-AAPM-pNA和琥珀酰-AAVA-pNA（琥珀酰，Suc；对硝基苯苯胺，pNA）的相对活性为100:56:32:3。
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