裂褶菌降解产物对酿酒酵母原生质体细胞再生的影响。

Effects of degraded schizophyllans on regeneration of protoplast cells of Saccharomyces cerevisiae.

作者信息

Hisamatsu M, Hayano M, Mishima T, Teranishi K, Yamada T

机构信息

Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Tsu, Japan.

出版信息

Biosci Biotechnol Biochem. 1995 Dec;59(12):2307-8. doi: 10.1271/bbb.59.2307.

DOI:10.1271/bbb.59.2307

PMID:8611754

Abstract

Schizophyllan was heated at 100 degrees C in 85% dimethyl sulfoxide (DMSO) containing 0.01 M H2SO4 for various times, and fractionated by gel-permeation chromatography. Molecular weights (M(r)) of the depolymerized products thus obtained were measured in water and DMSO by GPC-LALLS to estimate their conformations in water. The products with triple helical structure stimulated regeneration of Saccharomyces cerevisiae protoplast cells, while those of single chain conformation were totally inactive.

摘要

裂褶多糖在含0.01 M硫酸的85%二甲基亚砜（DMSO）中于100℃加热不同时间，然后通过凝胶渗透色谱进行分级分离。通过凝胶渗透色谱-激光光散射法（GPC-LALLS）在水和DMSO中测定由此获得的解聚产物的分子量（M(r)），以估计它们在水中的构象。具有三螺旋结构的产物刺激酿酒酵母原生质体细胞的再生，而单链构象的产物则完全无活性。