Hisamatsu M, Hayano M, Mishima T, Teranishi K, Yamada T
Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Tsu, Japan.
Biosci Biotechnol Biochem. 1995 Dec;59(12):2307-8. doi: 10.1271/bbb.59.2307.
Schizophyllan was heated at 100 degrees C in 85% dimethyl sulfoxide (DMSO) containing 0.01 M H2SO4 for various times, and fractionated by gel-permeation chromatography. Molecular weights (M(r)) of the depolymerized products thus obtained were measured in water and DMSO by GPC-LALLS to estimate their conformations in water. The products with triple helical structure stimulated regeneration of Saccharomyces cerevisiae protoplast cells, while those of single chain conformation were totally inactive.
裂褶多糖在含0.01 M硫酸的85%二甲基亚砜（DMSO）中于100℃加热不同时间，然后通过凝胶渗透色谱进行分级分离。通过凝胶渗透色谱-激光光散射法（GPC-LALLS）在水和DMSO中测定由此获得的解聚产物的分子量（M(r)），以估计它们在水中的构象。具有三螺旋结构的产物刺激酿酒酵母原生质体细胞的再生，而单链构象的产物则完全无活性。
