裂褶菌多糖与酵母葡聚糖的黏附作用及其对酵母原生质体再生的影响之间的相关性。
The correlation between adhesion of schizophyllan to yeast glucan and its effect on regeneration of yeast protoplast.
作者信息
Hisamatsu M, Mishima T, Teranishi K, Yamada T
机构信息
Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Tsu, Japan.
出版信息
Carbohydr Res. 1997 Feb 20;298(1-2):117-21. doi: 10.1016/s0008-6215(96)00299-6.
Schizophyllan, a water-soluble (1-->3)-beta-D-glucan with a triple-helical conformation, adheres to yeast glucan and curdlan gel. As the molecular weight of schizophyllan decreases, both its adhesion to the water-insoluble glucans and its ability to promote the regeneration of yeast protoplasts are reduced. Therefore, we hypothesize that schizophyllan can surround yeast protoplasts by adhering to a fragment of yeast glucan remaining or/and resynthesized on the cell surface and that this encapsulation allows regeneration of the protoplast cells to occur at very high frequency.
裂褶多糖是一种具有三螺旋构象的水溶性（1→3）-β-D-葡聚糖，它能黏附于酵母葡聚糖和凝胶多糖凝胶。随着裂褶多糖分子量的降低，其对水不溶性葡聚糖的黏附作用以及促进酵母原生质体再生的能力均会下降。因此，我们推测裂褶多糖可以通过黏附于细胞表面残留或/和重新合成的酵母葡聚糖片段来包围酵母原生质体，并且这种包封作用能使原生质体细胞以非常高的频率发生再生。
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