Beconi-Barker M G, Roof R D, Millerioux L, Kausche F M, Vidmar T H, Smith E B, Callahan J K, Hubbard V L, Smith G A, Gilbertson T J
Upjohn Company, Kalamazoo, MI 49001, USA.
J Chromatogr B Biomed Appl. 1995 Nov 17;673(2):231-44. doi: 10.1016/0378-4347(95)00258-1.
An HPLC method was developed and validated for the determination of ceftiofur-related metabolites that have the potential to be microbiologically active in swine muscle, kidney, liver and fat. Its performance was evaluated against incurred-residue swine tissues. This method is based on the cleavage of the disulfide and/or thioester bonds between the metabolites and their conjugate sulfur containing moiety using dithioerythritol to yield desfuroylceftiofur, and further stabilization to desfuroylceftiofur acetamide. The limit of quantitation was 0.1 micrograms ceftiofur equivalents/g tissue. The assay is specific for ceftiofur-related metabolites when evaluated against commercially available antibiotics for swine.
开发并验证了一种高效液相色谱法，用于测定猪肌肉、肾脏、肝脏和脂肪中具有微生物活性潜力的头孢噻呋相关代谢物。根据实际残留的猪组织评估了该方法的性能。该方法基于使用二硫苏糖醇裂解代谢物与其含硫共轭部分之间的二硫键和/或硫酯键，生成去呋喃甲酰头孢噻呋，并进一步稳定为去呋喃甲酰头孢噻呋乙酰胺。定量限为0.1微克头孢噻呋当量/克组织。当与市售猪用抗生素进行比较评估时，该测定法对头孢噻呋相关代谢物具有特异性。
