Wong Y C, Charles B G
Department of Pharmacy, University of Queensland, Brisbane, Australia.
J Chromatogr B Biomed Appl. 1995 Nov 17;673(2):306-10. doi: 10.1016/0378-4347(95)00268-4.
A simple, accurate and precise high-performance liquid chromatographic method is described for assaying lisinopril in human urine. Urine (1 ml) containing lisinopril and enalaprilat (internal standard) was acidified with 10 microliters of 6 M nitric acid, passed through a Sep-Pak C18 cartridge and eluted with 3 ml of 10% acetonitrile, followed by 6 ml of distilled water. the separations were carried out using a mu Bondapak c18 column with a mobile phase comprising acetonitrile (60 ml), methanol (10 ml) and tetrahydrofuran (10 ml) in 15 mM phosphate buffer (920 ml) at pH 2.90. Separations were performed at 40 degrees C and detection was at 206 nm. Standard calibration plots of lisinopril in urine were linear (r > 0.998) and recovery was greater than 64%. The lowest quantifiable concentration was 0.5 micrograms/ml. Within-day and between-day imprecision (coefficient of variation) ranged from 2.51% to 9.26%, and inaccuracy was less than 8.3%.
描述了一种用于测定人尿中赖诺普利的简单、准确且精密的高效液相色谱法。含有赖诺普利和依那普利拉（内标）的尿液（1 ml）用10微升6 M硝酸酸化，通过Sep-Pak C18柱，先用3 ml 10%乙腈洗脱，再用6 ml蒸馏水洗脱。分离在μ Bondapak c18柱上进行，流动相由60 ml乙腈、10 ml甲醇和10 ml四氢呋喃与15 mM磷酸盐缓冲液（920 ml）组成，pH为2.90。分离在40℃下进行，检测波长为206 nm。尿液中赖诺普利的标准校准曲线呈线性（r > 0.998），回收率大于64%。最低可定量浓度为0.5微克/毫升。日内和日间不精密度（变异系数）范围为2.51%至9.26%，误差小于8.3%。
