Pitzurra L, Rossetto O, Chimienti A R, Blasi E, Bistoni F
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Cell Immunol. 1996 Apr 10;169(1):113-6. doi: 10.1006/cimm.1996.0098.
The light chain of tetanus neurotoxin (TeTx) is a zinc endopeptidase specific for VAMP/synaptobrevin (VAMP), a 120-amino-acid integral protein previously described in the small synaptic vesicles of neuronal cells. TeTx has been shown to be active also on nonneuronal cells. By SDS-PAGE and quantitative immunoblotting on proteins derived from murine macrophages (Mphi) exposed to TeTx, we have shown that: (1) VAMP-related proteins are also present in Mphi and (2) such proteins are sensitive to TeTx proteolytic cleavage. The demonstration that TeTx acts on VAMP-related proteins also in Mphi offers a new and useful tool for molecular studies on Mphi exocytosis.
破伤风神经毒素（TeTx）的轻链是一种锌内肽酶，对VAMP/突触小泡蛋白（VAMP）具有特异性，VAMP是一种先前在神经元细胞的小突触小泡中描述的120个氨基酸的整合蛋白。TeTx已被证明对非神经元细胞也有活性。通过对暴露于TeTx的小鼠巨噬细胞（Mphi）来源的蛋白质进行SDS-PAGE和定量免疫印迹，我们已经证明：（1）Mphi中也存在VAMP相关蛋白；（2）这些蛋白对TeTx的蛋白水解切割敏感。TeTx在Mphi中也作用于VAMP相关蛋白这一发现为巨噬细胞胞吐作用的分子研究提供了一种新的有用工具。
