Treiber-Held S, Stewart D M, Kurman C C, Nelson D L
Immunophysiology Section, Metabolism Branch, National Cancer Institute, National Institutes of Health , Bethesda, Maryland 20982, USA.
Clin Immunol Immunopathol. 1996 Apr;79(1):71-8. doi: 10.1006/clin.1996.0052.
The recently identified and cloned cytokine IL-15 shares many of the T-cell and B-cell stimulatory activities of IL-2 and utilizes the beta and gamma chains of the IL-2R for binding and signaling. The present report shows that, like IL-2, IL-15 in a concentration and time-dependent manner causes the release of sIL-2Ralpha from PHA-activated human peripheral blood mononuclear cells. This effect of IL-15 is largely direct and independent of IL-2. Blocking of the IL-2Rbeta chain with the antibody Mik-beta1 prevented the release of sIL-2Ralpha by IL-15 but not by IL-2. IL-7, another cytokine utilizing the gamma chain of the IL-2R, drove the release of sIL-2Ralpha as well. Several clinical conditions are associated with abnormal serum sIL-2Ralpha levels and are also monitored by the measurement of sIL-2Ralpha. The reason for sIL-2Ralpha release is not fully understood. In this study, IL-15, like IL-2 was shown to be a potent inducer of sIL-2Ralpha release in vitro.
最近鉴定并克隆出的细胞因子白细胞介素-15（IL-15）具有许多与白细胞介素-2（IL-2）相同的T细胞和B细胞刺激活性，并利用IL-2受体的β链和γ链进行结合和信号传导。本报告显示，与IL-2一样，IL-15以浓度和时间依赖性方式促使PHA激活的人外周血单个核细胞释放可溶性IL-2受体α链（sIL-2Rα）。IL-15的这种作用在很大程度上是直接的，且不依赖于IL-2。用抗体Mik-β1阻断IL-2Rβ链可阻止IL-15诱导sIL-2Rα的释放，但不能阻止IL-2诱导的释放。另一种利用IL-2Rγ链的细胞因子IL-7也能促使sIL-2Rα的释放。几种临床病症与血清sIL-2Rα水平异常有关，并且也通过检测sIL-2Rα来进行监测。sIL-2Rα释放的原因尚未完全明确。在本研究中，IL-15与IL-2一样，在体外被证明是sIL-2Rα释放的有效诱导剂。
