Cossarizza A, Cooper E L, Suzuki M M, Salvioli S, Capri M, Gri G, Quaglino D, Franceschi C
Department of Biomedical Sciences, University of Modena, Italy.
Exp Cell Res. 1996 Apr 10;224(1):174-82. doi: 10.1006/excr.1996.0125.
Earthworm coelomocytes (leukocytes) effect cytotoxicity at significantly high levels against the NK-sensitive, human tumor cell line, K562, and the NK-resistant targets (U937, BSM, CEM). By cytofluorimetric analyses using mouse anti-human monoclonal antibodies and by morphological evaluations, two types of coelomocytes were identified: (1) small (8-11 micron) electron-dense cells (SC): CD11a+, CD45RA+, CD45RO+, CDw49b+, CD54+, beta 2-m+ and Thy-1+; (2) large (12-15 micron) electron-lucent cells (LC) that are negative for these markers. Both cell types were negative for other CD and MHC class I and class II markers. SC were active during recognition, rapidly binding to targets; LC were phagocytic. Release of 51Cr revealed rapid, significant, and equal levels of killing at 4 degrees, 20 degrees, and 37 degrees C. We propose that primitive NK-like activity appeared early in evolution.
蚯蚓体腔细胞（白细胞）对NK敏感的人肿瘤细胞系K562以及NK抗性靶细胞（U937、BSM、CEM）具有显著高水平的细胞毒性作用。通过使用小鼠抗人单克隆抗体的细胞荧光分析和形态学评估，鉴定出两种类型的体腔细胞：（1）小（8 - 11微米）电子致密细胞（SC）：CD11a +、CD45RA +、CD45RO +、CDw49b +、CD54 +、β2 - m +和Thy - 1 +；（2）大（12 - 15微米）电子透亮细胞（LC），这些标志物呈阴性。两种细胞类型对其他CD以及MHC I类和II类标志物均为阴性。SC在识别过程中活跃，能迅速与靶细胞结合；LC具有吞噬作用。51Cr的释放显示在4℃、20℃和37℃时杀伤迅速、显著且水平相当。我们认为原始的自然杀伤样活性在进化早期就已出现。
