Prijambada I D, Yomo T, Tanaka F, Kawama T, Yamamoto K, Hasegawa A, Shima Y, Negoro S, Urabe I
Department of Biotechnology, Faculty of Engineering, Osaka University, Japan.
FEBS Lett. 1996 Mar 11;382(1-2):21-5. doi: 10.1016/0014-5793(96)00123-8.
A library of artificial random proteins of 141 amino acid residues of which 95 are random and which includes the 20 kinds of amino acids was prepared. Out of the 25 identified random proteins, 5 were soluble in the cell lysate, indicating that about 20% of the random proteins expressed in Escherichia coli are expected to be soluble. The soluble random proteins RP3-42 and RP3-45 and insoluble RP3-70 were purified. The solubility of the purified form is the same as that in the cell lysate.
制备了一个人工随机蛋白质文库，其含有141个氨基酸残基，其中95个是随机的，并且包含20种氨基酸。在鉴定出的25种随机蛋白质中，有5种可溶于细胞裂解液，这表明预计在大肠杆菌中表达的随机蛋白质约有20%是可溶的。对可溶性随机蛋白质RP3 - 42和RP3 - 45以及不溶性的RP3 - 70进行了纯化。纯化形式的溶解度与在细胞裂解液中的溶解度相同。
