由于与疏水化聚阳离子络合，DNA与生物膜的亲和力增强。

DNA affinity to biological membranes is enhanced due to complexation with hydrophobized polycation.

作者信息

Yaroslavov A A, Sukhishvili S A, Obolsky O L, Yaroslavova E G, Kabanov A V, Kabanov V A

机构信息

Department of Polymer Sciences, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Russian Federation.

出版信息

FEBS Lett. 1996 Apr 15;384(2):177-80. doi: 10.1016/0014-5793(96)00281-5.

DOI:10.1016/0014-5793(96)00281-5

PMID:8612818

Abstract

The interaction of negatively charged liquid phosphatidylcholine/cardiolipin liposomes with water-soluble negatively charged DNA/cetylpyridinium bromide and DNA/poly(N-alkyl-4-vinylpyridinium bromide) complexes was studied. It is shown that the DNA/cetylpyridinium bromide complex while interacting with the liposomes is destroyed, so that the cetylpyridinium cation is incorporated into the liposomal membrane and DNA remains in the solution. The DNA/poly-(N-ethyl-4-vinylpyridinium bromide) complex does not interact at all with the liposomes. On the contrary, the complex of DNA with the poly(vinylpyridinium) cation carrying a small amount of N-cetyl groups is adsorbed on the membrane as a whole. The data obtained indicate that complexation of DNA with hydrophobized polycations can be used for enhancing DNA affinity to biological membranes.

摘要

研究了带负电荷的液态磷脂酰胆碱/心磷脂脂质体与水溶性带负电荷的DNA/十六烷基溴化吡啶和DNA/聚（N-烷基-4-乙烯基溴化吡啶）复合物之间的相互作用。结果表明，DNA/十六烷基溴化吡啶复合物与脂质体相互作用时会被破坏，使得十六烷基溴化吡啶阳离子被并入脂质体膜中，而DNA则留在溶液中。DNA/聚（N-乙基-4-乙烯基溴化吡啶）复合物根本不与脂质体相互作用。相反，带有少量N-十六烷基基团的聚（乙烯基吡啶）阳离子与DNA的复合物整体吸附在膜上。所得数据表明，DNA与疏水化聚阳离子的络合可用于增强DNA对生物膜的亲和力。