Casper R F, Meriano J S, Jarvi K A, Cowan L, Lucato M L
Department of Obstetrics and Genecology, University of Toronto, Ontario, Canada.
Fertil Steril. 1996 May;65(5):972-6. doi: 10.1016/s0015-0282(16)58271-5.
To determine the ability of the hypo-osmotic swelling test to select viable sperm from nonmotile sperm samples for intracytoplasmic sperm injection (ICSI).
Nonrandomized, sequential comparative study.
Thirteen couples enrolled in our ICSI program had 16 cycles in which sperm preparations with 0% motility were obtained. Five cycles used cryopreserved epididymal sperm with complete asthenozoospermia.
In eight cycles, the semen samples were washed through a Percoll gradient and sperm were selected randomly for ICSI. In another eight cycles, the washed sperm were placed in a hypo-osmotic solution (75 mM fructose; 25 mM sodium citrate dihydrate) and the sperm with curled tails taken up with the microinjection needle, rinsed, and used ICSI.
Fertilization rate per oocyte injected as determined by the presence of two pronuclei at 18 hours after retrieval and embryo cleavage rate per oocyte injected at 48 hours after retrieval.
With random sperm injection, the fertilization and cleavage rates were 26% and 23%, respectively. In contrast, after injection of sperm selected using the hypo-osmotic swelling test, fertilization and cleavage rates were significantly greater (43% and 39%, respectively). There were three pregnancies in the eight cycles with the hypo-osmotic swelling test-selected sperm, including two from frozen epididymal sperm.
Based on these preliminary observations, we believe that the hypo-osmotic swelling test will prove to be valuable for increasing fertilization and cleavage rates and pregnancy rates in ICSI cycles where no motile sperm are recovered.
确定低渗肿胀试验从无活力精子样本中筛选出有活力精子用于卵胞浆内单精子注射（ICSI）的能力。
非随机、序贯对照研究。
参与我们ICSI项目的13对夫妇进行了16个周期的治疗，这些周期中获取的精子样本活力为0%。其中5个周期使用了完全弱精子症的冷冻附睾精子。
在8个周期中，精液样本通过Percoll梯度洗涤，随机选择精子用于ICSI。在另外8个周期中，将洗涤后的精子置于低渗溶液（75 mM果糖；25 mM二水柠檬酸钠）中，用显微注射针吸取尾部卷曲的精子，冲洗后用于ICSI。
取卵后18小时观察到两个原核确定的每个注射卵母细胞的受精率，以及取卵后48小时每个注射卵母细胞的胚胎分裂率。
随机注射精子时，受精率和分裂率分别为26%和23%。相比之下，注射经低渗肿胀试验筛选的精子后，受精率和分裂率显著更高（分别为43%和39%）。在8个使用低渗肿胀试验筛选精子的周期中有3例妊娠，其中2例来自冷冻附睾精子。
基于这些初步观察结果，我们认为低渗肿胀试验对于提高未获得有活力精子的ICSI周期中的受精率、分裂率和妊娠率将被证明是有价值的。
