Wang C W, Lai Y M, Wang M L, Lee J D, Soong Y K
Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Linkou Medical Center, Taiwan, Republic of China.
J Reprod Med. 1997 Jul;42(7):448-50.
With the advent of intracytoplasmic sperm injection (ICSI), fertilization and pregnancy have been reported in complete asthenozoospermia. The major problem with the use of immotile sperm for ICSI has been differentiating between live and dead sperm. We successfully selected live sperms with the hypoosmotic swelling test, and a twin pregnancy resulted after intracytoplasmic injection of completely immotile sperm.
A 32-year-old woman and her 36-year-old husband had had primary infertility for four years. Evaluation of the couple showed complete asthenozoospermia of the husband. Ovulation in the wife was successfully stimulated with clomiphene citrate, follicle-stimulating hormone and human menopausal gonadotropin. We selected the viable sperm with the hypoosmotic swelling test. ICSI was then performed. After assisted hatching, we transferred four embryos on the third day after oocyte retrieval. Intrauterine twin pregnancy was confirmed by ultrasonography four weeks later. The healthy infants, free of anomalies, were born on the 36th gestational week.
The hypoosmotic swelling test is a simple method of selecting immotile and viable sperm for use in ICSI.
随着卵胞浆内单精子注射（ICSI）技术的出现，已有报道称在完全性弱精子症患者中实现了受精和妊娠。使用无活力精子进行ICSI的主要问题在于区分活精子和死精子。我们通过低渗肿胀试验成功筛选出活精子，并在卵胞浆内注射完全无活力精子后获得了双胎妊娠。
一名32岁女性及其36岁丈夫原发性不孕四年。对该夫妇的评估显示丈夫存在完全性弱精子症。使用枸橼酸氯米芬、促卵泡生成素和人绝经期促性腺激素成功刺激妻子排卵。我们通过低渗肿胀试验筛选出有活力的精子，随后进行了ICSI。在辅助孵化后，于取卵后第三天移植了4个胚胎。四周后经超声检查确认宫内双胎妊娠。健康婴儿于妊娠第36周出生，未发现异常。
低渗肿胀试验是一种为ICSI筛选无活力且有活力精子的简单方法。
