López A J
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh Pennsylvania 15213, USA.
Dev Biol. 1995 Dec;172(2):396-411. doi: 10.1006/dbio.1995.8050.
The production of transcription factor isoforms by developmentally regulated alternative splicing of pre-mRNAs is a widespread phenomenon. Frequently, differences in biochemical function among the isoforms can be predicted from sequence analysis, and in many instances such differences have been demonstrated in vitro or in cultured cells. A great variety of strategies for functional diversification can be classified into three main types: modulation of DNA binding specificity or affinity, production of activators and antagonists from the same gene, and modulation of dimerization properties. Despite obvious implications in many cases, the actual developmental consequences are understood only in a few instances. The roles inferred from these examples are diverse, ranging from developmental switches that have profound effects on pathways of differentiation to mechanisms that may optimize or fine-tune transcription factor function in different contexts.
通过对前体mRNA进行发育调控的可变剪接来产生转录因子异构体是一种普遍现象。通常，可以从序列分析中预测异构体之间生化功能的差异，并且在许多情况下，这种差异已在体外或培养细胞中得到证实。多种功能多样化策略可分为三种主要类型：调节DNA结合特异性或亲和力、从同一基因产生激活剂和拮抗剂以及调节二聚化特性。尽管在许多情况下有明显的影响，但仅在少数情况下了解其实际的发育后果。从这些例子中推断出的作用多种多样，从对分化途径有深远影响的发育开关到可能在不同背景下优化或微调转录因子功能的机制。
