空肠弯曲菌中一个编码与肽聚糖相关脂蛋白具有相似性的蛋白质（Omp18）的基因的克隆、测序及表达。
Cloning, sequencing, and expression of a gene from Campylobacter jejuni encoding a protein (Omp18) with similarity to peptidoglycan-associated lipoproteins.
作者信息
Konkel M E, Mead D J, Cieplak W
机构信息
Department of Microbiology, Washington State University, Pullman 99164, USA.
出版信息
Infect Immun. 1996 May;64(5):1850-3. doi: 10.1128/iai.64.5.1850-1853.1996.
Abstract
A Campylobacter jejuni genomic plasmid library was screened with antiserum generated against whole C. jejuni, revealing two immunoreactive clones. Sequence analysis of the recombinant plasmids revealed a common open reading frame of 498 nucleotides encoding a protein of 165 amino acids with a calculated molecular mass of 18,018 Da. The recombinant product partitioned to the outer membrane fractions of Escherichia coli transformants and has been designated Omp18. The deduced amino acid sequence of the cloned C. jejuni gene exhibits considerable similarity to peptidoglycan-associated lipoproteins from other gram-negative bacteria.
摘要
用针对空肠弯曲菌全菌产生的抗血清筛选空肠弯曲菌基因组质粒文库，发现了两个免疫反应性克隆。对重组质粒的序列分析揭示了一个498个核苷酸的共同开放阅读框，其编码一个由165个氨基酸组成的蛋白质，计算分子量为18,018道尔顿。重组产物定位于大肠杆菌转化体的外膜部分，并被命名为Omp18。克隆的空肠弯曲菌基因的推导氨基酸序列与其他革兰氏阴性菌的肽聚糖相关脂蛋白有相当大的相似性。
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本文引用的文献
1
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Mol Microbiol. 1995 Jun;16(6):1269-70. doi: 10.1111/j.1365-2958.1995.tb02348.x.
2
Kinetic and antigenic characterization of altered protein synthesis by Campylobacter jejuni during cultivation with human epithelial cells.空肠弯曲菌与人上皮细胞共培养期间蛋白质合成改变的动力学和抗原特性
J Infect Dis. 1993 Oct;168(4):948-54. doi: 10.1093/infdis/168.4.948.
3
The complete general secretory pathway in gram-negative bacteria.革兰氏阴性菌中的完整通用分泌途径。
Microbiol Rev. 1993 Mar;57(1):50-108. doi: 10.1128/mr.57.1.50-108.1993.
4
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Infect Immun. 1994 Sep;62(9):3633-9. doi: 10.1128/iai.62.9.3633-3639.1994.
5
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6
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