嗜肺军团菌上清液中缺乏类铁载体活性。
Absence of siderophore-like activity in Legionella pneumophila supernatants.
作者信息
Liles M R, Cianciotto N P
机构信息
Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois 60611, USA.
出版信息
Infect Immun. 1996 May;64(5):1873-5. doi: 10.1128/iai.64.5.1873-1875.1996.
Abstract
Conflicting reports have been given as to the existence of a Legionella pneumophila siderophore. Hence, we rigorously examined the reported siderophore-like activity using the chrome azurol S indicator. Although chrome azurol S reactivity was detected in supernatants, control experiments indicate that it was due to cysteine in the media. When bacteria were cultured in media without cysteine, no siderophores were detected.
摘要
关于嗜肺军团菌铁载体的存在，已有相互矛盾的报道。因此，我们使用铬天青S指示剂对报道的类似铁载体的活性进行了严格检测。尽管在上清液中检测到了铬天青S反应性，但对照实验表明这是由于培养基中的半胱氨酸所致。当细菌在不含半胱氨酸的培养基中培养时，未检测到铁载体。
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