一种在其他天然存在的氨基酸存在下直接测定半胱氨酸的分光光度法。
A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids.
作者信息
Gaitonde M K
出版信息
Biochem J. 1967 Aug;104(2):627-33. doi: 10.1042/bj1040627.
Abstract
- An acid ninhydrin reagent was found to react specifically in forming a pink product (E(max.) 560mmu) with cysteine. 2. The method was highly sensitive for the determination of cysteine (in 28.0x10(3)). Homocysteine, glutathione, proline, ornithine and other naturally occurring amino acids tested did not give a similar reaction. 3. The reaction product was stable for at least 3-4hr. at room temperature and the extinction was proportional to the concentration in the range 0.05-0.5mumole of cysteine. 4. The acid ninhydrin reagent also gave yellow products (E(max.) 370-404mmu) with tryptophan, 5-hydroxytryptophan, 5-hydroxytryptamine and indol-3-ylacetic acid. 5. The method was applied for the determination of cysteine in perchloric acid extracts of rat brain, liver and blood.
摘要
- 发现酸性茚三酮试剂与半胱氨酸特异性反应形成粉红色产物（最大吸收波长560nm）。2. 该方法对半胱氨酸的测定具有高度敏感性（检测限为28.0×10⁻³）。所测试的同型半胱氨酸、谷胱甘肽、脯氨酸、鸟氨酸及其他天然存在的氨基酸未产生类似反应。3. 反应产物在室温下至少3 - 4小时稳定，在0.05 - 0.5微摩尔半胱氨酸浓度范围内，吸光度与浓度成正比。4. 酸性茚三酮试剂与色氨酸、5 - 羟色氨酸、5 - 羟色胺及吲哚 - 3 - 乙酸也生成黄色产物（最大吸收波长370 - 404nm）。5. 该方法用于测定大鼠脑、肝和血液的高氯酸提取物中的半胱氨酸。
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