Yumita N, Umemura S, Magario N, Umemura K, Nishigaki R
School of Pharmaceutical Science, Toho University, Chiba, Japan.
Int J Radiat Biol. 1996 Mar;69(3):397-404. doi: 10.1080/095530096145959.
Sonodynamically induced lipid peroxidation with haematoporphyrin (Hp) was studied using rat erythrocytes. Both suspensions of erythrocyte ghosts and of intact erythrocytes were exposed to ultrasound in the same way in the presence and absence of Hp (80 microM). The lipid peroxidation in erythrocyte ghost membranes was estimated by measuring the amount of reactive substance produced from thiobarbituric acid added immediately after the exposure. Haematoporphyrin multiplied the ultrasonically induced lipid peroxidation by three to five times, while Hp alone showed no peroxidation. A 24-h interval between the exposure and the preparation for measurement did not increase the measured amount of peroxide. In the presence of Hp the estimated peroxidation rate and the rate of erythrocyte lysis correlated quite well for each acoustic condition and for each chemical condition such as the presence or absence of active oxygen scavengers in the suspension. The sonodynamically induced lipid peroxidation with Hp was doubled by deuterium oxide substitution for suspension medium and was significantly reduced by histidine, by sodium azide, and also by nitrogen substitution for saturation gas, whereas superoxide dismutase and mannitol showed no significant inhibitory effect. These results are consistent with a hypothesis that lipid peroxidation in membranes by singlet oxygen is the primary mechanism of sonodynamically induced erythrocyte lysis with Hp.
利用大鼠红细胞研究了血卟啉（Hp）介导的声动力学诱导的脂质过氧化作用。在存在和不存在Hp（80微摩尔）的情况下，以相同方式将红细胞影悬液和完整红细胞悬液暴露于超声下。通过测量暴露后立即添加的硫代巴比妥酸产生的反应性物质的量来估计红细胞影膜中的脂质过氧化作用。血卟啉使超声诱导的脂质过氧化作用增加了三到五倍，而单独的Hp未显示出过氧化作用。暴露与测量准备之间的24小时间隔并未增加所测量的过氧化物量。在存在Hp的情况下，对于每种声学条件以及每种化学条件（例如悬浮液中是否存在活性氧清除剂），估计的过氧化速率与红细胞裂解速率相关性良好。用重水替代悬浮介质使Hp介导的声动力学诱导的脂质过氧化作用增加了一倍，而组氨酸、叠氮化钠以及用氮气替代饱和气体均使其显著降低，而超氧化物歧化酶和甘露醇则未显示出显著的抑制作用。这些结果与单线态氧介导的膜脂质过氧化是Hp介导的声动力学诱导红细胞裂解的主要机制这一假设相一致。
