神经损伤增强大鼠神经元谷氨酸转运体表达：通过差异显示PCR鉴定
Nerve injury enhances rat neuronal glutamate transporter expression: identification by differential display PCR.
作者信息
Kiryu S, Yao G L, Morita N, Kato H, Kiyama H
机构信息
Department of Neuroanatomy, Osaka University Medical School, Japan.
出版信息
J Neurosci. 1995 Dec;15(12):7872-8. doi: 10.1523/JNEUROSCI.15-12-07872.1995.
An increase in neuronal glutamate transporter expression after nerve injury was demonstrated by means of differential display PCR (DD-PCR) coupled with in situ hybridization. DD-PCR was carried out to compare differences in expression of mRNAs between axotomized and normal hypoglossal motoneurons in the rat. The expression of several gene fragments were found to be increased following nerve injury; the full length cDNA corresponding to one fragment was cloned by subsequent rat cDNA library screening. The close homology of glutamate transporters with our rat cDNA led us to conclude that this clone corresponds to the rat neuronal glutamate transporter (rat EAAC1). We speculate that the upregulation of this glutamate uptake system may increase the resistance of these cells against neurotoxic glutamate accumulation during the process of nerve regeneration.
通过差异显示PCR（DD-PCR）结合原位杂交技术，证实了神经损伤后神经元谷氨酸转运体表达增加。采用DD-PCR比较大鼠轴突切断的舌下运动神经元和正常舌下运动神经元之间mRNA表达的差异。发现神经损伤后几个基因片段的表达增加；通过随后的大鼠cDNA文库筛选，克隆了与其中一个片段相对应的全长cDNA。谷氨酸转运体与我们的大鼠cDNA具有高度同源性，这使我们得出结论，该克隆对应于大鼠神经元谷氨酸转运体（大鼠EAAC1）。我们推测，这种谷氨酸摄取系统的上调可能会增加这些细胞在神经再生过程中抵抗神经毒性谷氨酸积累的能力。
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