Cytochemical specificity of acridine red towards RNA and depolymerised DNA.

The paper contains an account of the use of the basic dye, acridine red, of the xanthene group, for staining RNA and depolymerised DNA. The in situ absorption curve of nuclei stained with acridine red indicates a peak of maximum absorption at 560 nm, whereas in vitro absorption characteristics of an aqueous solution of the dye indicate the peak at 550nm. The possibility of using acridine red as a substitute for pyronin, when staining is required for RNA only or in methyl green-acridine red sequence for localising DNA and RNA has been discussed.