Beckers E A, Porcelijn L, Ligthart P, Vermey H, Von dem Borne A E, Overbeeke M A, van Rhenen D J
Red Cross Blood Bank and the Department of Hematology, University Hospital, Rotterdam, the Netherlands.
Transfusion. 1996 Feb;36(2):104-8. doi: 10.1046/j.1537-2995.1996.36296181919.x.
the RoHar antigenic complex has been characterized serologically by difficulties in D typing, weak e expression, lack of G antigen, presence of Rh33, a low-frequency Rh antigen, and, more recently, a second low-frequency antigen, FPTT. Allocation to one of the partial D catagories was not considered because of the unuaual reactions of RoHar cells and because anti-D production was not observed in RoHar persons.
Three unrelated RoHar donors and their families were studied in detail with special emphasis on D epitope mapping, e and G typing, and screening for antibodies.
Only D epitopes 5 and 6/7 were demonstrable, and D epitopes 1, 2, 3, 4, 8, and 9 seem to be absent in the RoHar complex. In one individual, the presence of alloanti-D with limited specificity, not reacting with RoHar red cells of other individuals, was found 6 months after a second D+ pregnancy.
The finding of alloanti-D in an RoHar r person supports the concept that the D characteristic of this phenotype is a partial D antigen, which is consistent with the presence of the limited number of D epitopes found in epitope mapping. As has been suggested for other partial D antigens, RoHar individuals should be regarded as D- for the receipt of blood, and pregnant RoHar women who have had D+ pregnancies should receive anti-D prophylaxis.
RoHar抗原复合物在血清学上的特征表现为D分型困难、e抗原表达弱、缺乏G抗原、存在低频Rh抗原Rh33，以及最近发现的第二种低频抗原FPTT。由于RoHar细胞的异常反应以及在RoHar个体中未观察到抗-D产生，因此未考虑将其归入部分D类别之一。
对三名无关的RoHar供者及其家族进行了详细研究，特别着重于D表位图谱分析、e和G分型以及抗体筛查。
仅可证明存在D表位5和6/7，而RoHar复合物中似乎不存在D表位1、2、3、4、8和9。在一名个体中，第二次妊娠D阳性6个月后，发现存在特异性有限的同种抗-D，该抗体不与其他个体的RoHar红细胞发生反应。
在一名RoHar r个体中发现同种抗-D支持了这一概念，即该表型的D特征是一种部分D抗原，这与表位图谱分析中发现的有限数量的D表位一致。正如针对其他部分D抗原所建议的那样，对于输血而言，RoHar个体应被视为D阴性，并且有过D阳性妊娠的RoHar孕妇应接受抗-D预防。
