Lomas C, Grässmann W, Ford D, Watt J, Gooch A, Jones J, Beolet M, Stern D, Wallace M, Tippett P
Medical Research Council Blood Group Unit, London, UK.
Transfusion. 1994 Jul;34(7):612-6. doi: 10.1046/j.1537-2995.1994.34794330017.x.
Several Rh D phenotypes with partial D antigens are recognized. Some partial D antigens are associated with low-incidence Rh antigens. New partial D antigens are revealed by an atypical pattern of reactions with anti-D.
The reactions of D variant cells with panels of monoclonal anti-D and with antibodies to low-incidence antigens were compared to those of known D categories to identify a new Rh D phenotype. The inheritance of partial D antigens was studied by Rh phenotyping of the families of the probands. Standard serologic methods were used and family data were analyzed.
A new Rh D phenotype, to be called DFR, was identified in 17 probands, two of whom had made anti-D. The partial D antigen carries epD3, epD4, and epD9 and lacks epD8. The presence of other D epitopes is ambiguous; different answers were obtained for the same sample with different monoclonal anti-D of the same apparent epitope specificity. The immunoglobulin class of the anti-D was important: IgG were more successful than IgM monoclonal anti-D in detecting the partial D of DFR. Family studies showed that DFR traveled with Ce more frequently than with cE. The low-incidence antigen FPTT (International Society of Blood Transfusion number 700048) was found on all DFR samples. Family studies demonstrated that FPTT is, as suspected, part of the complex Rh system.
The partial D of the Rh D phenotype, DFR, is recognized by its pattern of reactions with monoclonal anti-D and its association with the low-incidence antigen FPTT, FPTT has now been numbered Rh50.
已识别出几种带有部分D抗原的Rh D血型表型。一些部分D抗原与低发生率的Rh抗原相关。通过与抗-D的非典型反应模式揭示了新的部分D抗原。
将D变异细胞与单克隆抗-D组以及低发生率抗原抗体的反应与已知D类别细胞的反应进行比较，以鉴定一种新的Rh D血型表型。通过对先证者家族进行Rh血型分型研究部分D抗原的遗传情况。使用标准血清学方法并分析家族数据。
在17名先证者中鉴定出一种新的Rh D血型表型，命名为DFR，其中两人产生了抗-D。部分D抗原携带epD3、epD4和epD9，缺乏epD8。其他D抗原决定簇的存在尚不明确；对于具有相同表观抗原决定簇特异性的不同单克隆抗-D，同一个样本得到了不同的答案。抗-D的免疫球蛋白类别很重要：IgG单克隆抗-D在检测DFR的部分D方面比IgM单克隆抗-D更成功。家族研究表明，DFR与Ce一起出现的频率高于与cE一起出现的频率。在所有DFR样本上均发现了低发生率抗原FPTT（国际输血协会编号700048）。家族研究表明，正如所怀疑的那样，FPTT是复杂Rh系统的一部分。
Rh D血型表型DFR的部分D通过其与单克隆抗-D的反应模式及其与低发生率抗原FPTT的关联得以识别，FPTT现已编号为Rh50。
